葡萄籽原花青素对动脉粥样硬化兔血脂的调节作用

目的　研究葡萄籽原花青素 (GSP)对动脉粥样硬化(AS)兔血脂的调节作用。方法　 2 4只♂新西兰大白兔随机分为正常对照 (A)组、高脂模型 (B)组、GSP预防干预 (C)组 ,分别饲喂标准、标准 + 1%胆固醇、标准 + 1%胆固醇 + 1%GSP的颗粒饲料 ,于实验开始前 1d和开始后第 1、2、4、8、12wk末取空腹血 ,检测血清甘油三酯 (TG)、总胆固醇 (TC )、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C)。 12wk末取血后处死 ,取主动脉作病理形态学观察。所有数据经SAS 8 2进行混合模型的变异数分析。结果　与B组比较 ,C组TC、LDL C、TG和TG/HDL C在 12wk时降低 (P <0 0 1) ,HDL C升高 (P <0 0 5)。病理分析显示C组兔主动脉壁厚度和泡沫细胞数量明显较B组减轻 (P <0 0 1)。结论　GSP对血脂有调节作用 ,可通过降低TC、LDL C、TG、TG/HDL C和升高HDL C的作用而发挥抗AS作用 ,并有可能改善胰岛素抵抗状态

Effect of grape seed proanthocyanidin on the lipid profile of atherosclerosis in rabbits

AIMTo observe the effect of grape seed proanthocyanidi n (GSP) on the lipid profile of atherosclerosis in rabbits. METHODS24 male New Zealand rabbits were randomized into three groups with 8 rabbits in each group. They were individually housed in metal cages in an air-condition ed room. Throughout the experimental period, they were given restricted amounts of food (100～120 g/head per day) of each diet, with no limit of drinking. The c ontrol group (group A) were fed with standard diet. The cholesterol group (group B) was fed with standard diet containing 1% cholesterol. The GSP group (group C ) were fed with standard diet containing 1% cholesterol plus 1% GSP. All the rab bits were fed for twelve weeks. Blood samples were drawn from ear middle arterie s of rabbits just before the experiment and at the first, the second, the forth, the eighth and the twelfth weekend of the experiment. All the rabbits were fast ed for at least eight hours before the blood was drawn. The blood samples were a nalyzed for total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) , triglyceride (TG), and high density lipoprotein cholesterol (HDL-C) and they were measured enzymatically by automatic facilities. Aorta samples were observed through optical microscope. Results were expressed as means ±s. The Mixed Procedure of SAS 8 2 software was used to evaluate the differences betwee n the cholesterol group and the GSP group. RESULTSCompared with the group B, the TC,LDL-C,TG and the TG/HDL-C were lowered markedly by dietary GSP at the twelfth weekend (P<0 01), the HDL-C were improved markedly in the rabbits fed with GSP at the twelfth weekend (P<0 05). The extent and severity of atherosclerosis of the GSP gr oup were less than those of the cholesterol group, and the stability of the athe rosclerotic plaque of the GSP group was strengthened by GSP. CONCLUSION GSP has effect on the lipid profile, and this data demonstrates that GSP may provide significant health benefits by dramatically ameliorating the inc idence of atherosclerosis as demonstrated by reducing the TC, LDL-C, TG, TG/HDL -C and improving the HDL-C, and may have effect to improve insulin resistance.