|
|||||
|
|
| 细胞培养稳定同位素标记技术的发展及其在脑病研究中的应用 | |
| 引用本文： | 朱丽,刘安,杨洪军,陈畅,李德凤,吴传鸿,高健,李韶菁.细胞培养稳定同位素标记技术的发展及其在脑病研究中的应用[J].中国药理学通报,2012,28(7):897-900. |
| 作者姓名： | 朱丽 刘安 杨洪军 陈畅 李德凤 吴传鸿 高健 李韶菁 |
| 作者单位： | 1. 江西中医学院,江西,南昌,330006;中国中医科学院中药研究所,北京,100700 2. 中国中医科学院中药研究所,北京,100700 3. 河北大学药学院,河北,保定,071002 |
| 基金项目： | 国家自然科学基金资助项目(No 30901976);国家十二五“重大新药创制”科技重大专项资助项目(No2012ZX09103-201-055) |
| 摘 要： | 寻找脑中特异的蛋白标记物,对于脑病临床诊断及药物机制研究至关重要。人脑是一个复杂的有机体,采用单靶点、非动态、局部的方法不适于脑病研究,而以质谱技术为基础的定量蛋白质组学的出现和发展,加快了脑病标志分子的研究进程。细胞培养稳定同位素标记技术(SILAC)作为精确的蛋白定量方法,是系统、动态的研究方法,为脑病机制和治疗脑病中药的研究提供了新的思路。 |
| 关 键 词： | 定量蛋白质组学 SILAC 脑病 神经血管单元 中药 网络药理学 |
Development of stable isotope labeling technique and its application in encephalopathy research |
|
| ZHU Li , LIU An , YANG Hong-jun , CHEN Chang , LI De-feng , WU Chuan-hong , GAO Jian , LI Shao-jing.Development of stable isotope labeling technique and its application in encephalopathy research[J].Chinese Pharmacological Bulletin,2012,28(7):897-900. | |
| Authors: | ZHU Li LIU An YANG Hong-jun CHEN Chang LI De-feng WU Chuan-hong GAO Jian LI Shao-jing |
| Institution: | 1.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China; 2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 3.College of Pharmaceutical Science,Hebei University,Baoding Hebei 071002,China) |
| Abstract: | It is crucial to find out the brain specific protein markers for clinical diagnosis and drug mechanism.Human brain is a complex organism,so the single-target,non-dynamic and local method is not suitable for brain research.The development of quantitative proteomics,which is based on the mass spectrum,greatly accelerates the research process.As the accurate quantitative method of protein,the stable isotope labeling with amino acids in cell culture(SILAC) is a system,dynamic technology.It can provide new ideas for the mechanism research and the treatment strategy of traditional Chinese medicine in encephalopathy. |
| Keywords: | quantitative proteomics SILAC encephalopathy neurovascular unit traditional Chinese medicine network pharmacology |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |