| 神经生长因子抗体对哮喘模型大鼠肺中趋化素样因子1表达的影响 |
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| 引用本文： | 孔令雷,胡金凤,陈乃宏.神经生长因子抗体对哮喘模型大鼠肺中趋化素样因子1表达的影响[J].中国药理学通报,2012,28(6):757-760. |
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| 作者姓名： | 孔令雷 胡金凤 陈乃宏 |
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| 作者单位： | 天然药物活性物质与功能国家重点实验室,中国医学科学院北京协和医学院药物研究所,北京,100050 |
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| 基金项目： | 国家自然科学基金资助项目,国家创新药物重大专项资助项目 |
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| 摘 要： | 目的观察神经生长因子(NGF)抗体对OVA诱导的哮喘模型大鼠肺中趋化因子CKLF1的表达变化。方法采用OVA诱导的大鼠哮喘模型,通过腹腔给予NGF(8 ng.kg-1)及NGF抗体(0.1 mg.L-1),采用Western blot、ELISA和免疫组化观察CKLF1在支气管肺泡灌洗液(BALF)及肺组织中的表达变化。结果 CKLF1在OVA诱导的哮喘模型大鼠肺组织中的蛋白表达升高(P<0.05);NGF抗体能够降低CKLF1的表达;CKLF1在哮喘大鼠BALF中的含量升高(P<0.05),NGF抗体能够降低BALF中CKLF1的含量(P<0.05);NGF对肺组织及BALF中CKLF1的表达没有明显的影响。结论 NGF抗体能够抑制哮喘模型大鼠肺组织及支气管肺泡灌洗液中CKLF1的表达和释放,改善哮喘症状。
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| 关 键 词： | 趋化素样因子1 神经生长因子 哮喘 OVA 支气管肺泡灌洗液 表达定位 |
Effect of nerve growth factor antibody on the expression of chemokine-like factor 1 in asthma model of rats |
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| KONG Ling-lei
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HU Jin-feng
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CHEN Nai-hong.Effect of nerve growth factor antibody on the expression of chemokine-like factor 1 in asthma model of rats[J].Chinese Pharmacological Bulletin,2012,28(6):757-760. |
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| Authors: | KONG Ling-lei HU Jin-feng CHEN Nai-hong |
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| Institution: | (State Key Laboratory of Bioactive Substances and Functions of Natural Medicines,Dept of Pharmacology,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China) |
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| Abstract: | Aim To investigate the effect of nerve growth factor(NGF) antibody on the expression of chemokine-like factor 1(CKLF1) in asthma model of rats induced by OVA.Methods Elisa,western blot and immunohistochemistry were utilized to characterize the expression of CKLF1 in lung and bronchoalveolar lavage fluid of asthma rats induced by OVA after administration of NGF or NGF antibody.Results The expression of CKLF1 significantly increased in lung and bronchoalveolar lavage fluid of asthma rats,and treatment with NGF antibody significantly decreased the expression of CKLF1.In contrast,treatment with NGF did not increase the expression of CKLF1 in lung and bronchoalveolar lavage fluid.Conclusions Treatment with NGF antibody can inhibit the expression of CKLF1 in lung and bronchoalveolar lavage fluid and improve the asthma symptoms. |
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| Keywords: | chemokine-like factor 1 nerve growth factor asthma OVA bronchoalveolar lavage fluid expression and localization |
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