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木瓜苷对小鼠胶原性关节炎的预防作用及初步机制
引用本文:张玲玲,魏伟,严尚学,孙妩弋,岳莉,王华,吴成义,汪倪萍,李常玉.木瓜苷对小鼠胶原性关节炎的预防作用及初步机制[J].中国药理学通报,2004,20(1):95-100.
作者姓名:张玲玲  魏伟  严尚学  孙妩弋  岳莉  王华  吴成义  汪倪萍  李常玉
作者单位:安徽医科大学临床药理研究所,合肥,230032
基金项目:安徽省科技攻关项目,安徽省自然科学基金
摘    要:目的 研究木瓜苷 (GCS)预防用药对小鼠胶原性关节炎 (collagen inducedarthritis,CIA)的影响 ,并探讨其初步机制。方法 昆明种小鼠随机分为 6组 :正常对照组、模型组、木瓜苷三个剂量组和青藤碱组 (SIN) ;鸡Ⅱ型胶原免疫小鼠诱导继发性关节炎 ;足爪容积测定法、关节炎评分法和跖曲踝关节疼痛评分法观察关节炎的发生情况 ;观察胸腺指数、脾脏指数变化 ;3 H TdR参入法检测T、B细胞增殖反应 ;IL 1、IL 2活性的检测采用小鼠淋巴细胞增殖法 ;ELISA法检测血清中抗Ⅱ型胶原抗体水平 ;分光光度法测定局部踝关节PGE水平。结果 小鼠免疫后d 2 4 ,足爪出现红肿 ,继发性炎症高峰期在d 36 ,4 0后炎症逐渐减轻。GCS(6 0、12 0、2 4 0mg·kg-1)和SIN(10 0mg·kg-1)ig对CIA小鼠继发性关节炎有明显的抑制作用 ,降低CIA小鼠踝关节中增高的PGE ;CIA小鼠脾脏指数增加 ,而胸腺指数未见明显的改变 ,GCS能降低增加的CIA小鼠脾脏指数 ,对胸腺指数无明显影响 ;GCS(6 0、12 0、2 4 0mg·kg-1)和SIN (10 0mg·kg-1)使CIA小鼠增高的ConA和LPS诱导的T细胞和B细胞增殖反应降低 ;使T细胞和PMΦ分别产生的高水平IL 2和IL 1降至正常范围 ;并降低CIA小鼠血清中升高的抗CⅡ抗体。结论 GCS和SIN具有明显的抗炎作用 ,此作用可能是通过抑制

关 键 词:木瓜苷  胶原性关节炎  炎症  免疫
文章编号:1001-1978(2004)01-0095-06

Prophylactic effects of glucosides of cheanomeles speciosa on collagen-induced arthritis in mice
ZHANG Ling Ling,WEI Wei,YAN Shang Xue,SUN Wu Yi,YUE Li,WANG Hua,WU Cheng Yi,WANG Ni Ping,LI Chang Yu.Prophylactic effects of glucosides of cheanomeles speciosa on collagen-induced arthritis in mice[J].Chinese Pharmacological Bulletin,2004,20(1):95-100.
Authors:ZHANG Ling Ling  WEI Wei  YAN Shang Xue  SUN Wu Yi  YUE Li  WANG Hua  WU Cheng Yi  WANG Ni Ping  LI Chang Yu
Abstract:AIM To investigate the prophylactic effects of the glucosides of cheanomeles speciosa (GCS) on the collagen induced arthritis (CIA) in mice and its relative immunological mechanisms. METHODS Kunming mice were divided randomly into six groups including normal, model, GCS(60,120,240 mg·kg -1 ) and sinomenine (SIN) groups. Type Ⅱ collagen(CⅡ) induced arthritis model was built in mice. The effect of GCS in CIA mice was measured by paw swelling, arthritis scores and pain scores. Thymus indexes and spleens indexes were assayed. T and B cell proliferation, activity of interleukin 1(IL 1) produced by peritoneal macrophages(PMΦ) and interleukin 2(IL 2) produced by T cell were assayed by 3H TdR intake method. The level of anti CII IgG antibody in serum was assayed by ELISA. The level of PGE in ankle was assayed by ultraviolet spectrophotometer method. RESULTS The onset of paw swelling was on d 24 after injection of emulsion, the swelling peak appeared on d 36 and then declined after d 40. GCS (60,120,240 mg·kg -1 ×14 d) and SIN (100 mg·kg -1 ×14 d) significantly reduced paw swelling, decreased the arthritis scores and pain scores. Spleens indexes in CIA mice were increased, thymus indexes were not changed, GCS reduced elevatory spleens indexes of CIA mice, and suppressed the ConA or LPS induced T or B cell proliferation and production of IL 1 and IL 2 in CIA mice. Moreover GCS reduced the level of anti CII IgG antibody in serum and PGE in ankle of CIA mice. CONCLUSION GCS and SIN suppress the polyarthritis of CIA mice, suggesting that GCS has antiinflammatory effect on CIA mice. Its mechanism may be related to the modification of the abnormal immunological function of CIA mice.
Keywords:glucosides of cheanomeles speciosa  collagen  induced arthritis  inflammation  immune
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