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色胺酮对人白血病细胞株K562细胞增殖抑制、凋亡诱导的影响
引用本文:缪珊,张海,石小鹏,孙纪元,周宣暄,王捷频,苗青,谢艳华,王四旺.色胺酮对人白血病细胞株K562细胞增殖抑制、凋亡诱导的影响[J].中国药理学通报,2009,25(2).
作者姓名:缪珊  张海  石小鹏  孙纪元  周宣暄  王捷频  苗青  谢艳华  王四旺
作者单位:1. 第四军医大学药学系药物研究所,陕西,西安,710032
2. 第四军医大学实验动物中心,陕西,西安,710032
3. 第四军医大学西京医院药剂科,陕西,西安,710032
摘    要:目的探讨色胺酮(Tryptanthrin,Try)对人白血病细胞株K562细胞增殖和凋亡的影响。方法K562细胞进行常规培养后,利用MTT方法进行Try(1.56~50)mg.L-1的细胞增殖影响;Hoechst33258荧光染色观察细胞的形态学改变;流式细胞技术检测细胞周期及凋亡情况等指标;综合评价Try对K562细胞株增殖和凋亡的影响。结果Try在3.12~50mg.L-1浓度范围内能明显抑制K562细胞的增殖,并具有时间和浓度依赖性;Hoechst33258荧光染色及流式细胞仪结果显示,不同浓度Try作用48h后,K562细胞可发生明显的凋亡;同时Try也可影响K562的细胞周期,将细胞阻滞于G0/G1期,随Try剂量增大G0/G1期细胞比例也逐渐增高,并出现明显的亚二倍体凋亡峰;同时,S期细胞比例随剂量增大而逐渐降低。结论色胺酮能明显抑制K562细胞增殖并具有诱导K562细胞发生凋亡的作用。

关 键 词:色胺酮  K562细胞  细胞增殖  凋亡

Induction of apoptosis by Tryptanthrin on K562 cells
MIAO Shan,ZHANG Hai,SHI Xiao-peng,SUN Ji-yuan,ZHOU Xuan-xuan,WANG Jie-pin,MIAO Qing,XIE Yan-hua,WANG Si-wang.Induction of apoptosis by Tryptanthrin on K562 cells[J].Chinese Pharmacological Bulletin,2009,25(2).
Authors:MIAO Shan  ZHANG Hai  SHI Xiao-peng  SUN Ji-yuan  ZHOU Xuan-xuan  WANG Jie-pin  MIAO Qing  XIE Yan-hua  WANG Si-wang
Abstract:Aim To study the effect of Tryptanthrin(Try) on proliferation and apoptosis of erythroleukemia K562 cells.Methods The cell proliferation effect of Try(1.56~50 mg·L-1) on K562 cells was assessed by MTT assay.The morphologic change was observed by Hoechst 33258 fluore-scent stain.The flow cytometer was used to detect cell apoptosis and cell cycle.Results MTT showed that in the range of 3.12~50 mg·L-1 Try obviously inhibited the proliferation of K562 cells in a dose and time-dependent manner.Typical apoptosis changes were observed in K562 cells treated with Try for 48 h by flourescence inverted microscope.With Annexin V-FITC and PI double staining,folw cytometer result showed that the apoptosis state was obvious in K562 cells treated with 25,50 mg·L-1 Try for 48 h.The cell cycle distribution of K562 was changed.The G0/G1 phase was blocked and the DNA synthesis was inhibited,accompanied with subdiploid apoptotic peak.Conclusion Try has an effect on inhibiting the cell proliferation and inducing the apoptosis of K562.
Keywords:Tryptanthrin  K562 cell  cell proliferation  apoptosis
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