灯盏花素对慢性低氧大鼠PKC的影响

目的　探讨灯盏花素对慢性低氧大鼠PKC信号途径的影响。方法　将SD大鼠分为 :对照组 (A) ,低氧组 (B) ,低氧 +灯盏花素组 (C) ,低氧时间为 4wk。采用透射电镜、放射活性测定法、免疫组化等方法研究灯盏花素对慢性低氧大鼠肺动脉平均压 (mPAP)、左右心室重量比 (RV/LV +S)、肺细小动脉管壁面积 /管总面积 (WA/TA)、中膜平滑肌细胞核密度 (SMC)、肺细小动脉超微结构、肺组织PKC活性、肺细小动脉管壁PKC的影响。结果　 (1)B组mPAP、RV/LV +S明显高于A组 (P <0 0 1) ,C组mPAP、RV/LV +S明显低于B组 (P <0 0 1) ;(2 )光镜下B组WA/TA、SMC明显高于A组 (P <0 0 1) ,C组WA/TA、SMC明显低于B组 (P <0 0 1) ;电镜显示B组肺动脉中膜平滑肌细胞增生 ,胶原纤维丰富 ;C组肺动脉中膜平滑肌细胞、胶原纤维较B组明显减少 ;(3)B组肺组织PKC总活性 (PKCt)、胞膜PKC活性(PKCm)、胞质PKC活性 (PKCc)及胞膜PKC活性 (PKCm)占PKC总活性 (PKCt)的百分比明显高于A组 (P <0 0 1) ,C组PKCt、PKCm、PKCc及PKCm占PKCt的百分比明显低于B组 (P <0 0 1) ;(4 )免疫组化显示B组肺细小动脉 (直径约10 0～ 2 0 0 μm)PKC含量明显高于A组 (P <0 0 1) ,C组较B组明显为低 (P <0 0 1)。结论　灯盏花素抑制PKC信号途径可能是其抑制慢性低氧

Effect of breviscapine on protein kinase C of chronic hypoxic rats

AIM To study the effect of breviscapine on protein kinase C in pulmonary hypertensive rats induced by hypoxic. METHODS Thirty six rats were randomly divided into three groups: control group(A), hypoxic group(B),hypoxic+ breviscapine(bre.)group (C). The PKC activity of lung tissues were measured by radioactivity,the expression of PKC was observed in arterioles by immunohistochemistry. The average of integral light density (LD) of PKC in pulmonary arterioles was detected by image analysor and their relative content were calculated. RESULTS (1) Mean pulmonary arterial pressure (mPAP)and weight ratio of RV to LV+S of group B were significantly higher than those of group A( P< 0.01),mPAP and weight ratio of RV to LV+S of group C were significantly lower than those of group B ( P< 0.01); (2) WA/TA(vessel wall area/total area) and SMC(the density of medial smooth muscle cells) of pulmonary arterioles of group B were significantly higher those those of group A ( P< 0.01),WA/TA and SMC of pulmonary arterioles of group C were significantly lower than those of group B ( P< 0.01). Electron microscopy showed breviscapine could inhibit the proliferation of smooth muscle cells and the diposition of collgenous fiber in pulmonary arterioles induced by hypoxic. (3) The total, cytosolic and particulate fraction PKC activity and the ratio of particulate fraction to total PKC activity of group B were significantly higher than those of group A ( P< 0.01), the total, cytosolic and particulate fraction PKC activity and the ratio of particulate fraction to total PKC activity of group C were significantly lower than those of group B ( P< 0.01). (4) LD of PKC in pulmonary arterioles of group B was significantly higher than those of group A( P< 0.01), LD of PKC in pulmonary arterioles was significantly lower in rats of group C than that of group B( P< 0.01). CONCLUSION Breviscapine can inhibit pulmonary hypertension and structural remodeling of pulmonary arterials by inhibiting the effect of PKC.