首页 | 本学科首页   官方微博 | 高级检索  
检索        

藤茶总黄酮对拘束负荷引起小鼠肝损伤的保护作用
引用本文:郑洁静,续洁琨,江涛,赵亮,贺飞,姚新生,栗原博.藤茶总黄酮对拘束负荷引起小鼠肝损伤的保护作用[J].中国药理学通报,2006,22(10):1249-1253.
作者姓名:郑洁静  续洁琨  江涛  赵亮  贺飞  姚新生  栗原博
作者单位:1. 暨南大学中药及天然药物研究所,广东,广州,510632
2. 沈阳药科大学中药学院,辽宁,沈阳,110016
摘    要:目的研究藤茶总黄酮对拘束负荷造成小鼠肝损伤的保护作用。方法采用拘束负荷方法引起小鼠应激性肝损伤模型。赖氏法和TBARS法分别测定小鼠血浆中谷丙转氨酶活性和丙二醛含量,HPLC法测定肝组织匀浆中维生素C及谷胱甘肽水平,ORAC方法测定肝组织的抗氧化能力指数及藤茶总黄酮和二氢杨梅素的体外抗氧化活性。结果与拘束模型组相比,藤茶总黄酮可以明显提高拘束负荷小鼠肝组织匀浆的抗氧化能力指数、维生素C和谷胱甘肽水平,有效降低血浆中丙二醛含量和谷丙转氨酶水平。藤茶总黄酮及二氢杨梅素在体外显示出较强的抗氧化能力。结论藤茶总黄酮对拘束负荷引起的小鼠肝损伤具有一定的保护作用,其作用机制可能部分来自于清除自由基和抑制脂质过氧化过程。

关 键 词:藤茶总黄酮  拘束应激  肝损伤  抗氧化能力指数
文章编号:1001-1978(2006)10-1249-05
收稿时间:2006-04-13
修稿时间:2006-07-22

Protective effects of Tengcha flavonoids on liver injury in mice loaded with restraint stress
ZHENG Jie-jing,XU Jie-kun,JIANG Tao,ZHAO Liang,HE Fei,YAO Xin-sheng,Hiroshi Kurihara.Protective effects of Tengcha flavonoids on liver injury in mice loaded with restraint stress[J].Chinese Pharmacological Bulletin,2006,22(10):1249-1253.
Authors:ZHENG Jie-jing  XU Jie-kun  JIANG Tao  ZHAO Liang  HE Fei  YAO Xin-sheng  Hiroshi Kurihara
Abstract:Aim To observe the protective effects of Tengcha flavonoids(TCF) on liver injury in mice loaded with restraint stress.Methods The liver injury model was established under restraint stress for 12 h in mice.Hepatic function was evaluated by assessing alanine aminotransferase level in plasma.The levels of vitamin C and glutathione(GSH) were detected in liver by HPLC method.TBARS method was performed to detect the levels of Malondialdehyde(MDA) in plasma.Antioxidant capacity of liver was measured by oxygen radical absorbance capacity(ORAC) assay.Results TCF increased the levels of ORAC,vitamin C and GSH in liver,decreased the levels of ALT and MDA as compared with restraint stress control in a dose-dependent manner.Conclusion TCF exerted protective effects on liver injury in mice loaded with restraint stress,which may be related to its free radical scavenging activity.
Keywords:Tengcha flavonoids  restraint stress  liver injury  oxygen radical absorbance capacity
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号