斑蝥素对高转移卵巢癌细胞HO-8910PM中NF-κB(P65)、FAK表达及FAK磷酸化水平的影响

目的研究斑蝥素对HO8910PM细胞内NFκB(P65)、FAK表达及FAK磷酸化水平的影响,探讨其抗肿瘤侵袭转移的作用机制。方法MMT法测定斑蝥素对细胞增殖的影响;Westernblot分析NFκB(P65),FAK表达及FAK磷酸化水平。结果5～20μmol·L-1的斑蝥素作用HO8910PM细胞24h后,NFκB(P65)蛋白表达水平明显下降,并有一定的剂量效应关系;上调FAK表达,使FAK磷酸化水平降低。结论斑蝥素抗肿瘤侵袭转移与NFκB(P65)表达以及FAK磷酸化水平有关。

Effects of cantharidin on the expressions of NF-κB(P65),FAK protein and the level of phosphorylated FAK of highly metastatic ovarian carcinoma HO-8910PM cells

Aim To study the effect of cantharidin on the expression of NF-κB(P65), FAK protein and the level of phosphorylated FAK, and to investigate posssible mechanism of cantharidin on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells. Methods MTT assay was used to examine the effect of cantharidin on proliferation of HO-8910PM cells after 24 h treatment; The expression levels of NF-κB(P65), FAK protein and the level of phosphorylated FAK were assessed by Western blot analysis.Results The expression level of of NF-κB(P65) protein decreased obviously in HO-8910PM cells treated with 5～20 μmol·L -1 cantharidin for 24 h, and the effects appeared in a dose-dependent manner. Cantharidin significantly up-regulated the expression of FAK, down-regulated the level of phosphorylated FAK.Conclusion The antitumor activity of cantharidin is involved in the expression of NF-κB(P65) and the level of phosphorylated FAK.