首页 | 本学科首页   官方微博 | 高级检索  
检索        

盐酸埃他卡林对PD模型大鼠脑内突触体谷氨酸摄取的影响
引用本文:杨彦玲,孟长虹,丁建花,胡刚.盐酸埃他卡林对PD模型大鼠脑内突触体谷氨酸摄取的影响[J].中国药理学通报,2004,20(1):65-68.
作者姓名:杨彦玲  孟长虹  丁建花  胡刚
作者单位:南京医科大学药理及神经生物学教研室,南京,210029
摘    要:目的 研究谷氨酸转运体功能改变与帕金森病(Parkinson’sdisease ,PD)发病的相关性 ,探讨新型ATP敏感性钾通道 (ATPsensitivepotassiumchannel,KATP)开放剂盐酸埃他卡林 (Iptakalimhydrochloride ,Ipt)对PD模型大鼠脑内突触体摄取谷氨酸的影响及其机制。方法 采用 6 hydrox ydopamine(6 OHDA)建立PD大鼠模型 ,制备脑组织突触体 ;用同位素标记法测定L 3 H] glutamate摄取活性。 结果 PD模型大鼠纹状体和皮层的谷氨酸转运功能明显降低 ;Ipt(10、5 0和 10 0 μmol·L-1)具有恢复转运功能的作用 ,此作用可被KATP阻断剂Glibenclamide (2 0 μmol·L-1)逆转。结论 谷氨酸转运体功能下降与PD发病密切相关 ;Ipt通过激活KATP发挥促进谷氨酸摄取的作用 ,有望成为新一代PD治疗药物

关 键 词:盐酸埃他卡林  ATP敏感性钾通道  谷氨酸转运体  帕金森病
文章编号:1001-1978(2004)01-0065-04
修稿时间:2003年6月30日

Iptakalim hydrochloride enhances glutamate uptake activity in synaptosomes from rat models of Parkinsons disease
YANG Yan Ling,MENG Chang Hong,DING Jian Hua,HU Gang.Iptakalim hydrochloride enhances glutamate uptake activity in synaptosomes from rat models of Parkinsons disease[J].Chinese Pharmacological Bulletin,2004,20(1):65-68.
Authors:YANG Yan Ling  MENG Chang Hong  DING Jian Hua  HU Gang
Abstract:AIM To study the relationship between the activity of glutamate transporters and Parkinson's disease (PD), examine whether a novel ATP sensitive potassium (K ATP) channel opener Iptakalim (Ipt) hydrochloride enhances glutamate uptake activity, and to investigate its mechanisms. METHODS Rats were stereotaxically injected with 6 hydroxydopamine (6 OHDA) in SNpc. The synaptosomes from normal and PD rats were isolated, and 3H] glutamate uptake in synaptosomes was measured by using liquid scintillation counting. RESULTS 3H] glutamate uptake by synaptosomes from striatum and cerebral cortex of PD rats decreased and the redution was recovered by administration with Ipt (10, 50, 100 μmol·L -1 ). The protective effect of Ipt was blocked by co adiministration with glibenclamide (20 μmol·L -1 ), an inhibitor of sulphonylurea receptors. CONCLUSION Our results demonstrate for the first time the protective role of K ATP channel in glutamate uptake of synaptosomes and conceptually support the view that Ipt may have potential and feasibility in therapy for PD.
Keywords:iptakalim hydrochloride  ATP sensitive potassium channel  glutamate uptake  Parkinsons disease
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号