MitoQ对模拟高原缺氧大鼠脑组织的保护作用

目的研究MitoQ对模拟海拔8 000 m高原缺氧大鼠脑组织的保护作用。方法采用常压密闭缺氧实验考察MitoQ的抗缺氧活性,并确定最佳给药剂量;采用大型低压氧舱模拟海拔8 000 m高原缺氧环境,检测MitoQ对缺氧大鼠脑组织结构的影响,以及氧化应激及线粒体功能相关指标,同时检测凋亡相关蛋白,对MitoQ对缺氧大鼠的保护作用进行研究。结果MitoQ使模拟高原缺氧大鼠脑组织细胞间隙减小,空泡化程度降低,明显降低大鼠脑组织MDA含量(P<0.05),降低胞内Ga 2+含量(P<0.01),提高线粒体膜电位(P<0.05),在一定程度上提高缺氧条件下线粒体呼吸链复合物Ⅱ、Ⅳ的活性,明显降低Cyt C、caspase-3、Bax的表达(P<0.05,P<0.01),改善缺氧对大鼠脑及其线粒体功能的损伤。结论MitoQ可以减少模拟海拔8 000 m缺氧环境对大鼠造成的损伤,维持缺氧条件下大鼠脑组织线粒体功能,对缺氧条件下的大鼠发挥保护作用。

Protective effects of MitoQ against plateau hypoxia injury on rats

Aim To study the protective effects of MitoQ against an altitude of 8 000 m plateau hypoxia injury on brain tissues of rats. Methods We studied the protective effects of MitoQ against hypoxia by the experiment of closed normobaric hypoxia to choose the best dose firstly. Then, an altitude of 8 000 m plateau hypoxia environment was stimulated using a large scale hypobaric and mionectic chamber, the histopathological slices were observed, and the level of oxidative stress and mitochondrial function as well as measurement apoptosis related proteins were determined to study the anti-hypobaric hypoxia effects of MitoQ on rats. Results MitoQ reduced the intercellular space, the degree of vacuolization of brain and oxidative stress level, prevented calcium overload, raised mitochondrial membrane potential and increased the activity of mitochondrial respiratory chain complexes II, IV. Furthermore, MitoQ decreased the expression level of Cyt C, caspase-3 and Bax, playing a protective role under the condition of hypobaric hypoxia on rats. Conclusions MitoQ prevents the damage and maintains the function of mitochondria of brain to protect rats under the condition of simulated altitude of 8 000 m plateau hypoxia environment.