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雌激素及植物雌激素对去卵巢大鼠子宫组织VEGF表达和形态的影响
引用本文:杨丽娜,段颖,张英福,田治锋,邱小青,李红芳.雌激素及植物雌激素对去卵巢大鼠子宫组织VEGF表达和形态的影响[J].中国药理学通报,2009,25(8).
作者姓名:杨丽娜  段颖  张英福  田治锋  邱小青  李红芳
作者单位:1. 兰州大学基础医学院生理学教研室;甘肃省新药临床前研究重点实验室
2. 兰州大学基础医学院生理学教研室
3. 兰州大学基础医学院医学机能实验室,甘肃,兰州,730000
基金项目:兰州大学医学科研基金资助项目,甘肃省新药临床前研究重点实验室开放项目 
摘    要:目的比较雌激素及植物雌激素对去卵巢大鼠子宫组织VEGF和形态表达的影响。方法48只Wistar大鼠随机分为8组:假手术组(Sham)、去卵巢组(Ovx)、17β-雌二醇组(Ovx+Est)、17-β雌二醇+黄体酮组(Ovx+Est+Pro)、黄体酮组(Ovx+Pro)、染料木素组(Ovx+Gen)、白黎芦醇组(Ovx+Res)、根皮素组(Ovx+Phl)。给药21 d,用蛋白免疫印迹法观察各组子宫VEGF的表达、并用免疫组化对VEGF的表达部位和其形态学变化进行观察。结果与Sham组比较,Ovx组的VEGF表达下调;与Ovx比较,Ovx+Est和Ovx+Est+Pro两组VEGF表达均明显上调。Gen、Res和Phl对卵巢切除大鼠VEGF无影响。大鼠卵巢切除后,子宫明显萎缩且重量下降。皮下注射Est或Est合并Pro后,子宫组织密度增加,子宫被覆上皮细胞和腺体明显增生。皮下注射Gen和Phl后,子宫形态变化类似卵巢切除大鼠,注射Res可促进去卵巢大鼠子宫腺体的增生,但作用没有雌激素强。结论雌孕激素使VEGF表达明显上调;植物雌激素Res有促进腺泡增生作用。

关 键 词:去卵巢大鼠  雌激素  植物雌激素  孕激素  血管内皮生长因子  免疫印迹  免疫组化

Effects of estrogen and phytoestrogens on the VEGF expression and morphology in uterine tissue of ovariectomized rats
YANG Li-na,Duan Ying,ZHANG Ying-fu,TIAN Zhi-feng,QIU Xiao-qing,LI Hong-fang.Effects of estrogen and phytoestrogens on the VEGF expression and morphology in uterine tissue of ovariectomized rats[J].Chinese Pharmacological Bulletin,2009,25(8).
Authors:YANG Li-na  Duan Ying  ZHANG Ying-fu  TIAN Zhi-feng  QIU Xiao-qing  LI Hong-fang
Abstract:Aim To compare effects of estrogen with phytoestrogens on the VEGF expression and morphology in uterine tissue of ovariectomized rats.Methods 48 Wistar rats were randomly divided into 8 groups: Sham,Ovariectomized(Ovx),17β-estradiol(Ovx+Est),17β-estradiol+progesterone(Ovx+Est+Pro),progesterone(Ovx+Pro),Genistein(Ovx+Gen),resveratrol(Ovx+Res),phloretin(Ovx+Phl).After treated for 21 days,the expressions of VEGF were examined by the Western blot.The morphological changes and expression site in uterine tissues were observed by the immunohistochemical method.Results To compare Sham with Ovx group,the expression of VEGF was down-regulated in Ovx rats.However,to compare Ovx+Est,Ovx+Est+Pro with Ovx group respectively,their VEGF expressions were all up-regulated significantly.Gen,Res and Phl had no obvious effect on the expression of uterine VEGF in Ovx rats.After bilateral ovariectomy,the rat uterine atrophied.And after treatment with Est and Est+Pro,the uterine had obvious proliferation and its histological density increased.After injected Gen and Phl subcutaneously,the Ovx rat uterine had no change in morphology,but administration of Res could proliferate the uterine alveoli cell.Conclusions The expressions of VEGF are up-regulated in Est and Est+Pro groups.Res can proliferate the uterine alveoli cell.
Keywords:ovariectomized rats  estrogen  phytoestrogen  progesterone  vascular endothelial growth factor  Western blot  immunohistochemistry
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