大豆异黄酮对去卵巢大鼠骨密度和雌激素活性的影响(英文)

目的 :研究大豆异黄酮对去卵巢大鼠骨密度以及雌激素活性的影响。方法 :将 10～ 12月龄的雌性Wistar大鼠随机分为 6组 :假手术组 (SHAM )、切卵巢模型组 (OVX)、尼尔雌醇组 (OVX E)、小剂量异黄酮组 (L ISO)、中剂量异黄酮组 (M ISO)、大剂量异黄酮组 (H ISO) ,每组 8只。后 5组大鼠被切除双侧卵巢 ,SHAM组只被切除卵巢附近脂肪组织。L ISO ,M ISO ,H ISO分别灌胃给予 30 ,6 0 ,12 0mg·kg- 1的大豆异黄酮 ,OVX E组大鼠灌胃给予0 .2mg·kg- 1·wk- 1的尼尔雌醇 ,SHAM与OVX组以等剂量的溶剂灌胃 ,15wk后股动脉放血处死动物 ,收集血液用于血清碱性磷酸酶活性、血清雌二醇水平、血钙、血磷测定 ,分离出右侧股骨、第 2腰椎用于骨密度测量 ,测定双侧子宫重量。结果 :与SHAM组相比 ,OVX组股骨骨密度和椎骨骨密度均可见不同程度降低 (P <0 .0 5和P >0 .0 5 ) ,尼尔雌醇与异黄酮 12 0mg·kg- 1能明显升高去卵巢大鼠的股骨和椎骨骨密度 (P <0 .0 5 )。OVX组的血清碱性磷酸酶水平高于SHAM组 (P <0 .0 5 ) ,异黄酮6 0mg·kg- 1可降低去卵巢大鼠的血清碱性磷酸酶水平 (P <0 .0 5 )。OVX组的血清雌二醇水平与子宫系数明显低于SHAM组 (P <0 .0 1) ,异黄酮的不同剂量组与OVX E组的血清雌二醇水平、子宫系数都明显高于OV

Effects of soy isoflavones on bone density and estrogen activity in ovariectomized rats

AIM: To study effects of soy isoflavones on estrogen-like activity and bone density in ovariectomized adult rats. METHODS: Forty-eight female Wistar rats (10-12 mo old) were randomly divided into six groups. Ovariectomies were performed in the OVX (ovariectomy), OVX-E(OVX+nilestriol), L-ISO (OVX+soy isoflavones 30 mg*kg-1), M-ISO(OVX+soy isoflavones 60 mg*kg-1) and H-ISO(OVX+soy isoflavones 120 mg*kg-1) groups and animals in the SHAM group were subject to sham operation. Soy isoflavones 30,60,120 mg*kg-1 were given in the L-ISO, M-ISO and H-ISO groups respectively, nilestriol 0.2 mg*kg-1*wk-1 was given in OVX-E group, and the vehicle was given in SHAM and OVX groups. The drugs were all oral administered using a stomach tube. RESULTS: Bone mineral density of right femur was lower in OVX group than those in SHAM, H-ISO and OVX-E groups (P<0.05), there was a tendency of bone density in lumbar vertebra to be lower in OVX group than that in SHAM group. H-ISO and OVX-E rats had higher bone density of lumbar vertebra than that in OVX rats (P<0.05). Serum alkaline phosphates (ALP) activities were higher in OVX group than those in SHAM and M-ISO groups (P<0.05). The OVX group had lower serum estrogen level than those of all other groups. Although uterus relative weights in L-, M- and H-ISO groups were all lower than those in SHAM and OVX-E groups, they were higher than that in the OVX group. CONCLUSION: Soy isoflavones might exert estrogen-like effect in ovariectomized rats. Soy isoflavones 120 mg*kg-1 was effective in preventing bone loss induced by ovariectomy. This result suggests that other mechanisms may involve in this bone protective effect in addition to its estrogenic activity.