| HPLC法测定小儿咽扁颗粒中绿原酸的含量 |
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| 引用本文： | 邵秀芬,蔡盛.HPLC法测定小儿咽扁颗粒中绿原酸的含量[J].黑龙江医药,2010,23(6):887-889. |
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| 作者姓名： | 邵秀芬 蔡盛 |
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| 作者单位： | 杭州容立医药科技有限公司,杭州,310012 |
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| 摘 要： | 目的：采用高效液相色谱法建立小儿咽扁颗粒中绿原酸的含量测定方法。方法：依利特Hypersil ODS柱（4.6mm×250mm,5μm）,以乙腈-0.2%磷酸溶液（10∶90）为流动相,检测波长为327nm。结果：绿原酸在1.016～101.6μg/ml之间线性关系良好（γ=1）,回收率为98.57%（RSD为0.55%）。结论：该方法操作简单,分离效果好,可作为小儿咽扁颗粒的质量控制标准。
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| 关 键 词： | 小儿咽扁颗粒 绿原酸 含量测定 高效液相色谱法 |
Determination of Chlorogenic Acid of Xiao`er Yanbian Granules by HPLC |
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| Institution: | Shao Xiufen,et al (Hangzhou SFFT Developing Co,.Ltd.,,Hangzhou 310012) |
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| Abstract: | Objective：An HPLC method for the determination of Chlorogenic acid in Xiao＇er Yanbian Granules was established.Methods：A column of Hypersil ODS was used with acetonitrile and 0.2% solution of phosphoric acid（10：90）as the mobile phase.The detection wavelength was 327nm.Results：The linear range was 1.016～101.6μg/ml,γ=1,and average recovery was 98.57% with RSD 0.55%.Conclusion：The method is simple and good separation efficiency.It can be used in the standard of Xiao＇er Yanbian Granules. |
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| Keywords: | Xiao＇er Yanbian Granules Chlorogenic acid content determination HPLC |
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