| 虾青素促进丙烯酰胺所致大鼠海马神经细胞损伤的恢复 |
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| 引用本文： | 曹秀明,樊宇,宋冬雪,李钧.虾青素促进丙烯酰胺所致大鼠海马神经细胞损伤的恢复[J].中国海洋药物,2015,34(3):9-13. |
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| 作者姓名： | 曹秀明 樊宇 宋冬雪 李钧 |
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| 作者单位： | 哈尔滨商业大学 生命科学与环境科学研究中心 哈尔滨150076,哈尔滨商业大学 生命科学与环境科学研究中心 哈尔滨150076,哈尔滨商业大学 生命科学与环境科学研究中心 哈尔滨150076,哈尔滨商业大学 生命科学与环境科学研究中心 哈尔滨150076 |
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| 基金项目： | 黑龙江省教育厅科研项目(12541197)资助 |
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| 摘 要： | 目的从细胞水平上研究虾青素对丙烯酰胺所致海马神经细胞损伤恢复的影响。方法通过B27无血清培养法进行SD乳鼠的大脑海马神经细胞的分离、培养及其鉴定;MTT法检测细胞存活率,比色法测定细胞内抗氧化酶SOD、CAT、GSH-Px的活性,线粒体的MDA及NO的含量。结果虾青素能提高丙烯酰胺损伤过的海马神经细胞的存活率,提高了丙烯酰胺损伤后细胞SOD、CAT活性、GSH-Px的活力,并降低了细胞线粒体中的MDA和NO的含量。结论虾青素对丙烯酰胺造成的高海马神经细胞损伤有促进恢复的作用。
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| 关 键 词： | 虾青素 丙烯酰胺 海马神经细胞 氧化损伤 |
| 收稿时间： | 1/4/2015 12:00:00 AM |
| 修稿时间： | 2015/2/12 0:00:00 |
Astaxanthin Promotes Acylamide- damaged Hippocampal Neurons to Restore Damage |
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| CAO Xiu-ming,FAN Yu,SONG Dong-Xue and LI Jun.Astaxanthin Promotes Acylamide- damaged Hippocampal Neurons to Restore Damage[J].Chinese Journal of Marine Drugs,2015,34(3):9-13. |
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| Authors: | CAO Xiu-ming FAN Yu SONG Dong-Xue and LI Jun |
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| Institution: | Research Centre of Life Sciences and Environment Sciences,Harbin University of Commerce,Research Centre of Life Sciences and Environment Sciences,Harbin University of Commerce,Research Centre of Life Sciences and Environment Sciences,Harbin University of Commerce,Research Centre of Life Sciences and Environment Sciences,Harbin University of Commerce |
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| Abstract: | Objective To investigate the effects of Astaxanthin on the acylamide-damaged Hippocampal Neurons. Methods The Hippocampal Neuron isolated from SD neonatal rats,cultured by B27 serum-free medium and identified by NSE immunohistochemistry assay. MTT assay was used to examine the cell viability. The activity of SOD, CAT, GSH-Px was determined with Colorimetric methods as well as the content of MDA and NO of chondriosome in the Hippocampal Neuron. Results Astaxanthin improves the cell viability of acyamide-damaged Hippocampal Neurons, and the activity of SOD, CAT and GSH-Px as well as reduces the content of MDA and NO of chondriosome. Conclusion: Astaxanthin promotes Acylamide- damaged Hippocampal Neurons to Restore Damage. |
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| Keywords: | Astaxanthin acrylamide hippocampal neurons oxidative damage |
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