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D-氨基葡糖盐酸盐对胃癌细胞SGC-7901细胞周期及相关蛋白表达的影响
引用本文:曹秀明,张珍珠,王佳.D-氨基葡糖盐酸盐对胃癌细胞SGC-7901细胞周期及相关蛋白表达的影响[J].中国海洋药物,2008,27(6).
作者姓名:曹秀明  张珍珠  王佳
作者单位:哈尔滨商业大学生命科学与环境科学研究中心,国家教育部抗肿瘤天然药物工程研究中心,黑龙江,哈尔滨,150076
基金项目:黑龙江省教育厅科学研究基金 
摘    要:目的 研究D-氨基葡萄糖盐酸盐诱导人胃癌细胞SGC-7901的凋亡作用,并探讨其作用机制.方法 分别采用SRB法测定D-氨基葡萄糖盐酸盐对SGC-7901细胞的生长抑制作用,流式细胞仪观察对细胞周期及细胞色素C表达的影响,激光共聚焦显微镜检测细胞内Ca2+浓度的变化,Western Blot法测定周期蛋白B1、钙调神经磷酸酶表达的变化.结果 D-氨基葡萄糖盐酸盐能够引起SGC-7901细胞周期产生S期阻滞,细胞内Ca2+浓度增加,周期蛋白B1表达下降、钙调神经磷酸酶及细胞色素C的表达升高.结论 D-氨基葡萄糖盐酸盐使SGC-7901细胞周期发生阻滞并诱导细胞凋亡.

关 键 词:D-氨基葡萄糖盐酸盐  胃癌细胞SGC-7901  细胞周期  凋亡

Effects of D-Glucosamine hydrochloride on the cell-cycle and expression of proteins in gastric carcinoma cell SGC-7901
CAO Xiu-ming,ZHANG Zheng-zhu,Wang jia.Effects of D-Glucosamine hydrochloride on the cell-cycle and expression of proteins in gastric carcinoma cell SGC-7901[J].Chinese Journal of Marine Drugs,2008,27(6).
Authors:CAO Xiu-ming  ZHANG Zheng-zhu  Wang jia
Institution:CAO Xiu-ming,ZHANG Zheng-zhu,Wang-jia (Institute of Material Medical,Harbin University of Commerce Ministry of Education Engineering Research Center,Harbin 150076,China)
Abstract:Objective To study the effects of D-glucosamine hydrochloride on the apoptosis of gastric carcinoma cell line SGC-7901 and its mechanism.Methods SRB assay was used to examine the effects of D-glucosamine hydrochloride on the growth inhibition of SGC-7901.The cell-cycle and expression of cytochrome-C were observed by flow cytometry,the concentrations of intracellular Ca2+ was determined by CLSM and the expressions of cyclinB1,calcinerin were investigated by Western-Blot.Results D-glucosamine hydrochloride co...
Keywords:D-Glucosamine hydrochloride  gastric carcinoma SGC-7901  cell-cycle  apoptosis  
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