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多肋藻(Costaria costata)多糖的提取分离及理化性质分析
引用本文:李苗苗,于广利,吴建东,王培培,赵峡,段德麟.多肋藻(Costaria costata)多糖的提取分离及理化性质分析[J].中国海洋药物,2011,30(1):1-5.
作者姓名:李苗苗  于广利  吴建东  王培培  赵峡  段德麟
作者单位:海洋药物教育部重点实验室山东省糖科学与糖工程重点实验室中国海洋大学;中国科学院海洋研究所;
基金项目:国家自然科学基金(31070724); 长江学者和创新团队发展计划(IRT0944); 国家海洋公益性行业科研专项基金(201005024); 山东省农业良种工程重点项目(KSCX2-YW-N-47-02); 中国科学院知识创新工程项目资助
摘    要:目的 从多肋藻(Costaria costata)中提取分离多糖并对其进行理化性质分析.方法 采用水和2%碳酸钠水溶液于85℃提取多糖,采用高效凝胶渗透色谱法(HPGPC)分析多糖相对分子质量,用核磁共振氢谱(<'1>H-NMR)法分析褐藻胶中M/G比,用高效液相色谱法(HPLC)分析褐藻糖胶单糖组成.结果 从多肋藻中...

关 键 词:多肋藻  褐藻胶  褐藻糖胶  理化性质

Extraction,isolation and physicochemical analysis of polysaccharides from Costaria costata
LI Miao-miao,YU Guang-li,WU Jian-dong,WANG Pei-pei,ZHAO Xia,DUAN De-lin.Extraction,isolation and physicochemical analysis of polysaccharides from Costaria costata[J].Chinese Journal of Marine Drugs,2011,30(1):1-5.
Authors:LI Miao-miao  YU Guang-li  WU Jian-dong  WANG Pei-pei  ZHAO Xia  DUAN De-lin
Institution:LI Miao-miao1,YU Guang-li1,WU Jian-dong1,WANG Pei-pei1,ZHAO Xia1,DUAN De-lin2(1.Key Laboratory of Marine Drugs,Ministry of Education,Key Laboratory of Glycoscience andGlycoengineering of Shandong Province,Ocean University of China,Qingdao 266003,China,2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)
Abstract:Objective To extract polysaccharides from Costaria costata and analyse their physicochemical characters.Methods The polysaccharides were extracted with water and 2% sodium carbonate water solution at 85 ℃,their relative molecular masses(Mr) were analyzed by high performance gel permeation chromatography(HPGPC),the M/G ratio of alginate was determined by 1H-NMR,and the monosaccharides composition of fucoidans were determined by high performance liquid chromatography(HPLC).Results The polysaccharides of alginate(ALG) and two kinds of fucoidan(CWF,CAF) were extracted from C.costata with the yield of 42.4%,3.32% and 3.52%,respectively.Their Mr were 212,335 and 195 kD,respectively.The M/G ratio of ALG was 1.55.There were seven kinds of monosaccharides in CWF and CAF,and the molar ratio of fucose,galactose,mannose,glucuronic acid,rhamanose,xylose and glucosamine were 50.54,20.53,10.90,8.87,2.67,4.30,2.20 and 47.52,32.10,7.90,4.28,3.70,1.20,3.30,respectively.The FTIR spectra,both CWF and CAF showed specific absorption at 1235 cm-1,which indicate the existance of sulfate in the polysaccharides,and the sulfate contents of CWF and CAF were 23% and 9%,respectively.Conclusion The polysaccharides in C.costata were mainly composed by alginate and two kinds of structural complex fucoidans.
Keywords:Costaria costata  alginate  fucoidan  physicochemical characters  
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