中国南海侧扁软柳珊瑚Subergorgia suberosa次级代谢产物

目的研究中国南海侧扁软柳珊瑚Subergorgia suberosa的次级代谢产物。方法利用硅胶柱层析、Sephadex LH-20凝胶柱层析、HPLC等方法对化合物进行分离纯化;利用NMR,MS等波谱方法,并与文献对照,鉴定化合物的结构;利用抗菌活性模型和卤虫致死活性模型评价化合物的生物活性。结果从侧扁软柳珊瑚中分离鉴定了6个单体化合物,分别为:倍半萜化合物subergorgic acid(1),2-β-hydroxysubergorgic acid(2)和2-β-acetoxysubergorgic acid(3);甾体化合物3β-hydroxy-5α-pregnan-20-one(4),5α-pregnane-3,20-di-one(5)和胆甾醇(6)。结论化合物1～3对白色葡萄球菌Staphylococcus albus均显示出较强的抑制活性,化合物4对蜡状芽孢杆菌Bacillus cereus显示出较强的抑制活性,而化合物4和5显示出较强的卤虫致死活性。

Secondary metabolites of gorgonian Subergorgia suberosa from the South China Sea

Objective To investigate the secondary metabolites of gorgonian coral Subergorgia suberosa.Methods Column chromatography on silica gel and Sephadex LH-20,and semi-preparative HPLC were used to isolate and purify the secondary metabolites.The structures of isolated compounds were finally identified by spectroscopic analysis including NMR and MS,and by comparison with the data of literature.The bioactivities of the compounds were evaluated with antibacterial and brine shrimp lethality models.Results Six compounds were isolated from S.suberosa,and their structures were determined as three sesquiterpenes subergorgic acid(1),2-β-hydroxysubergorgic acid(2) and 2-β-acetoxysubergorgic acid(3),and three steroids: 3β-hydroxy-5α-pregnan-20-one(4),5α-pregnane-3,20-dione(5) and cholesterol(6).Conclusion Compounds 1~3 exhibited potent antibacterial activity against Staphylococcus albus,and 4 exhibited antibacterial activity against Bacillus cereus.Compounds 4 and 5 showed brine shrimp lethality toward Artemia salina.