| 扇贝糖胺聚糖的琼脂糖凝胶电脉分析 |
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| 引用本文： | 王长云,管华诗,刘树青.扇贝糖胺聚糖的琼脂糖凝胶电脉分析[J].中国海洋药物,1996,15(2). |
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| 作者姓名： | 王长云 管华诗 刘树青 |
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| 作者单位： | 青岛海洋大学海洋药物与食品研究所，青岛海洋大学海洋药物与食品研究所，青岛海洋大学海洋药物与食品研究所 青岛 266003，青岛 266003，青岛 266003 |
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| 摘 要： | 从海湾扇贝边中提取分离出扇贝糖胺聚糖,其红外光谱与肝素类似。将扇贝糖胺聚糖制品在0.06mol/L,pH8.6巴比妥缓冲系统中进行琼脂糖凝胶电泳分析,发现其电泳图谱显示单一区带;与糖胺聚糖标准品——肝素、透明质酸和6-硫酸软骨素对照,其电泳相对迁移率与肝素量为接近。
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| 关 键 词： | 扇贝 糖胺聚糖 琼脂糖凝胶电泳 分析 |
IDENTIFICATION OF GLYCOSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIAUS BY AGAROSE GEL ELEC-TROPHORESIS |
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| Wang Changyun,Guan Huashi,Liu Shuqing.IDENTIFICATION OF GLYCOSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIAUS BY AGAROSE GEL ELEC-TROPHORESIS[J].Chinese Journal of Marine Drugs,1996,15(2). |
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| Authors: | Wang Changyun Guan Huashi Liu Shuqing |
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| Abstract: | Fraction F3-3-3 of glycosaminoglycan was isolated from the leftover bits of Bay scallop Argopecten irradiaus . Infrared spectra of F3-3-3 was similar to that of heparin. Agarose gel electrophoresis of this fraction was performed in the system of barbital buffer (0. 06 mol/L,pH 8. 6). It was found that F3-3-3 showed single band in the electrophoresis. Compared with the agarose gel electrophoresis of standard glycosaminoglycan, such as heparin, hyaluronic acid and chondroitin 6-sulfate, it indicated that the relative migration rate of the fraction was close to that of heparin. |
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| Keywords: | Bay scallop (Argopecten irradiaus ) glycosaminoglycan agarose gel elec-trophoresis analysis |
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