南海深海沉积环境来源真菌Aspergillus flavus SCSIO F025的次生代谢产物及活性研究

目的 对采集自南海中部1781 m沉积环境的1株曲霉属真菌Aspergillus flavus SCSIO F025进行次生代谢产物及活性研究。方法 通过优化培养条件对菌株进行规模发酵,发酵产物采用硅胶柱层析、Sephadex LH-20、HPLC等色谱学方法进行化学分离,利用NMR、MS等波谱学技术并结合文献进行化合物的结构鉴定,应用纸片扩散法及DPPH自由基清除法对化合物进行初步抗氧化和抗菌活性测试。结果 从菌株SCSIO F025中分离鉴定5个单体化合物：penicillivinacine(1),arthrographol(2),dehydroxypaxilline(3),ditryptophenaline(4),kojic acid(5)。其中化合物1和2为首次从黄曲霉中分离得到。化合物1对溶藻弧菌和藤黄微球菌有弱的抑制活性,化合物2表现出显著抗氧化及溶藻弧菌和藤黄微球菌抑制活性,化合物3对溶藻弧菌和肺炎克雷伯杆菌有抑制活性。

Study on the Secondary Metabolites from South China Sea Deep-sea Sediment Derived Fungus Aspergillus flavus SCSIO F025

Objective To study the chemical constituents and bioactivities of Aspergillus flavus SCSIO F025, a deep-sea sediment derived fungus from South China Sea. Methods Through screening the fermentation conditions, the crude extracts were purified by silica gel column, Sephadex LH-20, and semi-preparative HPLC. The isolated compounds were identified by spectroscopic analysis (NMR and MS) as well as comparison with literature. They were tested for antioxidative and antibacterial activities by using Kirby-Bauer test and DPPH radical scavenging method. Results Five compounds were obtained from the ethyl acetate extracts of strain SCSIO F025. Their structures were identified as penicillivinacine (1), arthrographol (2), dehydroxypaxilline (3), ditryptophenaline (4), kojic acid (5). Compounds 1 and 2 were isolated from Aspergillus flavus for the first time. Compound 1 showed inhibitory activity against Vibrio alginolyticus and Micrococcus luteus. Compound 2 showed significant activity against DPPH and inhibition of Vibrio alginolyticus and Micrococcus luteus activity. Compound 3 showed inhibitory activity against Vibrio alginolyticus and Klebsiella pneumonia.