海洋硫酸多糖聚古罗糖酯(912)抗肿瘤作用机理探讨

目的：研究海洋硫酸多糖聚古罗糖酯(912)的抗肿瘤作用机理。方法：以S180荷瘤小鼠为模型，研究912体内对S180肉瘤生长的影响；MTT法观察912对肿瘤细胞ts-FT210体外增殖的影响；流式细胞仪PI染色法检测912对肿瘤细胞ts-FT210增殖周期的影响；FITC染色法测定其对ts-FT210蛋白质合成的影响；荧光染色和竞争抑制实验探讨肿瘤细胞ts-FT210上912结合位点的存在。结果：912体内能明显抑制S180肉瘤的生长，体外能抑制肿瘤细胞ts-FT210的增殖，将其押制在S和G2／M期，能抑制其蛋白质的合成，且912能与肿瘤细胞ts-FT210发生结合，但并不引起该肿瘤细胞的凋亡。结论：海洋硫酸多糖912可通过周期押制、蛋白质合成抑制实现其抗肿瘤活性。

A study on antitumor mechanisms of marine sulfated polysac-charide poly-guluronic acid (912)

Objective To study the antitumor mechanisms of marine sulfated polysaccharide po-ly-guluronic acid(912). Methods Effect of 912 on the growth of sarcoma 180 in vivo was evaluated on the sarcoma 180 of mice. Effect of 912 on the proliferation of ts-FT210 cells in vitro was evaluated by MTT assay, and effects on cell-cycle and synthesis of proteins of ts-FT210 cells were assessed by flow cytometry. The existence of binding sites of 912 on ts-FT210 cells was confirmed by fluorescent staining and competitive binding assay. Results 912 inhibited the sarcoma 180 growth in vivo. 912 inhibited the synthesis of proteins and the proliferation of ts-FT210 cells during the S and G2/M phases of cell division, but couldn't induce the apoptosis of ts-FT210 cells and there existed binding sites of 912 on ts-FT210 cells. Conclusion 912 exerted its inhibitory effect on the proliferation of ts-FT210 cells by cell-cycle inhibiting and protein synthesis inhibiting.