| 新疆产甘草6个不同地理群体遗传关系的RAPD分析 |
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| 引用本文： | 吴霞,刘庆华,马永红,党荣理.新疆产甘草6个不同地理群体遗传关系的RAPD分析[J].中国生化药物杂志,2003,24(4):191-193. |
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| 作者姓名： | 吴霞 刘庆华 马永红 党荣理 |
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| 作者单位： | 1. 新疆药物研究所,新疆,乌鲁木齐,830002
2. 新疆军区联勤部,卫生防疫大队,新疆,乌鲁木齐,830011 |
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| 基金项目： | 国家重点科技攻关项目 (2 0 0 0 K0 1 0 5 0 2 ) |
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| 摘 要： | 目的探讨新疆产甘草不同地理群体的遗传多样性。方法用人工栽培品种作为对照 ,采用 2 0个 10碱基随机引物对新疆产甘草 (GlycyrrhizauralensisFisch .exDC) 6个不同地理群体的基因组DNA进行RAPD分析。结果共扩增出 175个位点 ,其中多态性位点 10 2条 ,占 5 8.2 8%。通过计算遗传相似性系数 ,建立了UPGMA聚类图。结论不同地理群体中存在一定的遗传多样性 ;产地相距越远 ,群体间相似性程度越低 ;人工栽培种与同一产地野生种具有相似的遗传特性
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| 关 键 词： | DNA随机扩增多态性 甘草 遗传多样性 新疆 |
| 文章编号： | 1005-1678(2003)04-0191-03 |
| 修稿时间： | 2002年9月9日 |
RAPD analysis of genetic relationship among different populations of Glycyrrhiza uralensis Fisch.ex DC from six growing area in Sinkiang |
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| Fisch.ex DC from six growing area in Sinkiang WU Xia ,LIU Qing-hua ,MA Yong-hong ,DANG Rong-li.RAPD analysis of genetic relationship among different populations of Glycyrrhiza uralensis Fisch.ex DC from six growing area in Sinkiang[J].Chinese Journal of Biochemical Pharmaceutics,2003,24(4):191-193. |
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| Authors: | Fischex DC from six growing area in Sinkiang WU Xia LIU Qing-hua MA Yong-hong DANG Rong-li |
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| Institution: | Fisch.ex DC from six growing area in Sinkiang WU Xia 1,LIU Qing-hua 1,MA Yong-hong 2,DANG Rong-li 2 |
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| Abstract: | PurposeTo study the genetic relationship of Glycyrrhiza uralensis Fisch.ex DC from six growing areas in Sinkiang.MethodsTwenty different 10 bp oligonucleotide primers of arbitrary sequence were used to amplify the DNAs of Glycyrrhiza uralensis Fisch.ex DC from six growing areas in Sinkiang, using cultivated species as control.ResultsAmplified results showed total record of 175 bands, among which 102 (58.28%) were polymorphic. By calculating the genetic similarity coefficient, UPGMA clustering map was made.ConclusionThe genetic diversity exists in different local populations. The far the growing area,the lower the comparability degree among the populations. The wild species and the cultivated species in the same growing area had similar genetic characteristic. |
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| Keywords: | RAPD Glycyrrhiza uralensis Fisch ex DC genetic diversity sinkiang |
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