| 壳聚糖珠固定化乳糖酶的条件及特性研究 |
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| 引用本文： | 姜梅,王善荣.壳聚糖珠固定化乳糖酶的条件及特性研究[J].中国生化药物杂志,2006,27(2):92-94. |
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| 作者姓名： | 姜梅 王善荣 |
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| 作者单位： | 南京农业大学,食品科技学院,江苏,南京,210095 |
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| 基金项目： | 南京农业大学校科研和教改项目 |
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| 摘 要： | 目的研究乳糖酶的固定化。方法以壳聚糖珠(2.0 g)为载体,0.02%戊二醛为交联剂,考察固定化乳糖酶的条件及其特性。结果固定化条件:室温状态下,在pH 6.0、酶量0.2 mg/mL的酶液中固定12 h,固定化乳糖酶活力回收率为31.5%。乳糖酶、固定化乳糖酶的最适pH值分别为5.0~5.5,5.5~6.5;最适温度分别为40℃,50℃。结论固定化乳糖酶最适温度和pH值比溶乳糖高。
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| 关 键 词： | 乳糖酶 固定化 壳聚糖珠 特性 |
| 文章编号： | 1005-1678（2006）02-0092-03 |
| 收稿时间： | 2005-06-01 |
| 修稿时间： | 2005-11-28 |
Properties of immobilized galactosidase on chitosan beads |
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| JIANG Mei,WAN Shan-rong.Properties of immobilized galactosidase on chitosan beads[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(2):92-94. |
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| Authors: | JIANG Mei WAN Shan-rong |
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| Institution: | Food and Science Technology School of Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | PurposeThe Galactosidase was immobilized on glutaradehyde-pretreated chitosan beads.MethodsThe galactosidase was immobilized on glutaradehyde-pretreated chitosan beads,and the optimum conditions and properties of immobilized galactosidase were investigated.ResultsThe optimum conditions of galactosidase immobilized on glutaradehyde-pretreated chitosan beads were 0.02% glutaradehyde,pH 6.0,12 h and 0.2 mg/mL of the enzyme concentration at the room temperature.The optimum pH value for immobilized galactosidase(IGOS)and galactosidase(GOS) were 5.5-6.5,5.0-5.5,respectively.The optimum temperature for IGOS and GOS was 50℃and 40℃,respectively.ConclusionThe optimum pH and temperature of IGOS are higher than those of GOS. |
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| Keywords: | galactosidase immobilzation chitosan beads properties |
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