| 山楂中谷甾醇抑制肿瘤细胞的研究 |
| |
| 引用本文： | 董贺,张太平,李俊,彭士明,张鹤云.山楂中谷甾醇抑制肿瘤细胞的研究[J].中国生化药物杂志,2009,30(4). |
| |
| 作者姓名： | 董贺 张太平 李俊 彭士明 张鹤云 |
| |
| 作者单位： | 南京大学,生命科学院,医药生物技术国家重点实验室,江苏,南京,210093 |
| |
| 摘 要： | 目的从山楂中提取及鉴定谷甾醇,并研究其对3种肿瘤细胞和人体正常肝脏细胞的作用。方法取对数生长期的细胞进行MTT法细胞增殖实验及DNA凝胶电泳实验,观察谷甾醇对不同细胞的作用。结果MTT法细胞增殖实验显示一定剂量的谷甾醇对体外培养的HepS、S180、EAC细胞有显著的抑制作用(P<0.01),抑制率总体上与时间和浓度呈正相关;对L02细胞无明显的抑制作用(P>0.05);电泳结果显示经谷甾醇作用的HepS细胞DNA出现梯带。结论谷甾醇可能是通过促进肿瘤细胞凋亡来抑制其增殖。
|
| 关 键 词： | 山楂 谷甾醇 抗肿瘤 DNA梯带 |
The study on inhibition of tumour cells with sitosterol from hawthorn fruits |
| |
| DONG He,ZHANG Tai-ping,LI Jun,PENG Shi-ming,ZHANG He-yun.The study on inhibition of tumour cells with sitosterol from hawthorn fruits[J].Chinese Journal of Biochemical Pharmaceutics,2009,30(4). |
| |
| Authors: | DONG He ZHANG Tai-ping LI Jun PENG Shi-ming ZHANG He-yun |
| |
| Abstract: | Purpose To extract and identify sitosterol from hawthorn fruits to study its anticancer effect on several cells.Methods Designing experiments of cells proliferation and DNA agarose gel electrophoresis.Results The proliferation experiments showed that sitosterol has an inhibiting effect on HepS,S180,EAC cell(P<0.01),except L02 cells.And the inhibitory ratehad a direct ratio to the value of time and concentration.DNA ladders were found in DNA agarose gel electrophoresis results with HepS cells.Conclusion Sitosterol can inhibit proliferation of cancer cells,probably by causing apoptosis. |
| |
| Keywords: | hawthorn fruits sitosterol anticancer effect DNA ladder |
| 本文献已被 万方数据 等数据库收录！ |
|
