| D-氨基葡萄糖盐酸盐诱导淋巴细胞增殖及其机制 |
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| 引用本文： | 曹秀明,马娇,万晓峰.D-氨基葡萄糖盐酸盐诱导淋巴细胞增殖及其机制[J].中国生化药物杂志,2009,30(4). |
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| 作者姓名： | 曹秀明 马娇 万晓峰 |
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| 作者单位： | 哈尔滨商业大学,生命科学与环境科学研究中心药物所,抗肿瘤天然药物教育部工程研究中心,黑龙江,哈尔滨,150076 |
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| 基金项目： | 黑龙江省科技厅自然科学基金 |
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| 摘 要： | 目的研究D-氨基葡萄糖盐酸盐(GAH)对小鼠淋巴细胞增殖及其作用机制。方法利用荧光染料CFSE为细胞标记,研究GAH对小鼠淋巴细胞增殖的影响。激光共聚焦显微镜、流式细胞仪分别检测GAH对细胞内Ca2+浓度、钙调蛋白(CaM)表达的影响。结果GAH可使淋巴细胞增殖。加入GAH 2,4,6 h后,细胞内Ca2+浓度与对照组相比显著升高。GAH作用72 h后,细胞内CaM阳性表达细胞的百分率与对照组相比显著增加,由(61.80±0.97)%增至(79.21±0.95)%,说明GAH能够诱导淋巴细胞内CaM表达增加。结论GAH能够提高淋巴细胞内Ca2+浓度,促进CaM表达,触发Ca2+信号通路,活化淋巴细胞增殖。
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| 关 键 词： | D-氨基葡萄糖盐酸盐 淋巴细胞增殖 Ca2+ 钙调蛋白 |
Effect of D-glucosamine hydrochloride on lymphocytes proliferation and its mechanism |
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| CAO Xiu-ming,MA Jiao,WAN Xiao-feng.Effect of D-glucosamine hydrochloride on lymphocytes proliferation and its mechanism[J].Chinese Journal of Biochemical Pharmaceutics,2009,30(4). |
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| Authors: | CAO Xiu-ming MA Jiao WAN Xiao-feng |
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| Abstract: | Purpose To investigate the effect of D-glucosamine hydrochloride(GAH) on lymphocytes proliferation and its mechanism.Methods Fluorochrome label,scanning microscope and flow cytometry were used to investigate the effects of GAH on lymphocytes proliferation,intracellular Ca2+ concentration and expression of calmodulin(CaM).Results GAH can induce lymphocytes proliferation 2,4 and 6 h after being treated with GAH,and intracellular Ca2+ concentration was stepped up 72 h after being treated with GAH.The percentage of intracellular calmodulin expression was increased by GAH.Conclusion GAH can increase the intracellular Ca2+,incourage calmodulin expression and trigger Ca2+ signal pathway and activate lymphocytes proliferation. |
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| Keywords: | Ca2+ |
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