敷脐巴布剂的制备及对大鼠胃肠动力与胃肠激素的影响

目的观察敷脐巴布剂对胃手术模型大鼠胃肠运动和胃肠激素的影响,探讨其可能的作用机制。方法①观察胃手术模型大鼠用敷脐巴布剂敷脐后碳素墨水在小肠的移动长度,并与巴布剂基质组、手术模型组进行比较;②用放射免疫分析法测定巴布剂敷脐10 d后大鼠小肠组织胃动素(MOT)和生长抑素(SS)的水平。结果①敷脐巴布剂组能明显提高碳素墨水在大鼠小肠的推进距离与百分比,与巴布剂基质组及手术模型组比较差异显著(P<0.01),巴布剂基质组与手术模型组无显著差异(P>0.05);②敷脐巴布剂可提高大鼠小肠组织MOT及降低小肠组织SS水平,与手术模型组比较差异显著(P<0.05或P<0.01)。结论敷脐巴布剂能促进胃手术模型大鼠术后胃肠蠕动,对胃肠激素水平有调节作用。

Umbilical cataplasm preparation and effects on rat gastrointestinal motility and gastrointestinal hormones

Purpose To observe the effects of umbilical cataplasm on rat gastrointestinal motility and gastrointestinal hormones using rat model of gastric operation,and to explore its possible mechanism.Methods ①The peristalsis length of carbon ink in small intestine of 20 rats was investigated by the use of umbilical cataplasm,compared with the matrix cataplasm group and the untreated control group. ②By means of radioimmunoassay,the levels of motilin(MOT) and somatostatin(SS) in small intestine tissue of 20 rats were investigated after a ten-day course of application of umbilical cataplasm.Results ①The group of umbilical cataplasm could significantly increase the peristalsis length of carbon ink in the rat small intestine and the distance percentage to the whole intestine,compared with the matrix cataplasm group and the untreated control group has significant difference(P＜0.01),and has no significant differernce between the other two groups(P＞0.05). ②Umbilical cataplasm can increase the levels of MOT and decrease the levels of SS in small intestine tissue of rat,compared with the untreated group P＜0.05 or P<0.01.Conclusion Umbilical cataplasm can promote gastrointestinal peristalsis of the rat model of gastric operation, and can regulate the levels of gastrointestinal hormones.