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甲状旁腺素1-34肽的固相合成
引用本文:周贺钺,修朝阳,陈常庆.甲状旁腺素1-34肽的固相合成[J].中国生化药物杂志,2002,23(3):109-111.
作者姓名:周贺钺  修朝阳  陈常庆
作者单位:中国科学院,上海生物工程研究中心,上海,200233
基金项目:国家新药开发基金资助项目 ( 96 90 1 0 5 185)
摘    要:目的以Wang树脂为载体 ,应用芴甲氧羧基 /tBu方法合成人甲状旁腺素。方法芴甲氧羧基保护氨基酸用 1 氧 3 双二甲胺羧基苯骈三氮唑四氟化硼盐 / 1 羧基苯并三氮唑活化后 ,进行缩合反应。合成的多肽用三氟乙酸处理 ,从Wang树脂上切除。所得粗肽用SP Sepharose FF色谱柱和半制备型高效液相色谱分离纯化。结果用反相高效液相色谱法和毛细管电泳法鉴定纯度在 92 %以上。通过电喷雾质谱法测定其分子量为 4 1 1 6D ,与计算值相符。总收率为 8%。结论此工艺简单 ,易于扩大 ,适合于规模化生产

关 键 词:固相合成  Wang树脂  芴甲氧羧基氨基酸  甲状旁腺素
文章编号:1005-1678(2002)03-0109-03
修稿时间:2001年7月2日

Solid-phase synthesis of N-terminal 1-34 peptide of human parathyroid hormone
ZHOU He yue,XIU Zhao yang,CHEN Chang qing.Solid-phase synthesis of N-terminal 1-34 peptide of human parathyroid hormone[J].Chinese Journal of Biochemical Pharmaceutics,2002,23(3):109-111.
Authors:ZHOU He yue  XIU Zhao yang  CHEN Chang qing
Abstract:PurposeThe human parathyroid hormone 1 34 peptide was synthesized by Fmoc/tBu method out on wang resin.MethodsIn coupling step, Fmoc amino acid was activated with TBTU/HoBt.After the completion of peptide elongation, and the crude peptide product was cleaved out from Wang resin by TFA treatment. Then it was purified with SP Sepharose FF and semi preparative HPLC.ResultsThe purity of final product confirmed by HPLC and capillary zone electrophoresis to be more than 92%. Electrospray mass spectrometry analysis showed the right molecular weight of the synthetic peptide. The total yield was 8%.ConclusionThe technology process was simple. It could be enlarged easily and suitable to large scale manufacturing.
Keywords:solid phase synthesis  wang resin  Fmoc amino acid  parathyroid hormone  
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