| 牛凝血酶的制备研究 |
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| 引用本文： | 钱珊珊,高学军,张强,莫继先.牛凝血酶的制备研究[J].中国生化药物杂志,2006,27(6):346-348. |
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| 作者姓名： | 钱珊珊 高学军 张强 莫继先 |
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| 作者单位： | 东北农业大学,生命科学学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 目的从牛血浆中制备高活力及高收率的凝血酶。方法以牛血为原料,采用柠檬酸钡吸附、硫酸铵分级沉淀、DEAE-纤维素色谱等方法制备凝血酶原,并模拟生理条件用凝血酶原活酶复合物,包括牛凝血因子Ⅴ、Ⅹa、磷脂和Ca2+对凝血酶原进行激活。结果采用此方法制备的凝血酶的比活力为85 U/mg,收率为135μg/mL血浆。结论采用此制备技术,可以大规模制备高活力及高收率的牛凝血酶。
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| 关 键 词： | 凝血因子 凝血酶原活酶复合物 牛凝血酶 制备 |
| 文章编号： | 1005-1678（2006）06-0346-03 |
| 收稿时间： | 2006-01-24 |
| 修稿时间： | 2006-03-03 |
Preparation of thrombin in bovine blood |
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| QIAN Shan-shan,GAO Xue-jun,ZHANG Qiang,MO Ji-xian.Preparation of thrombin in bovine blood[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(6):346-348. |
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| Authors: | QIAN Shan-shan GAO Xue-jun ZHANG Qiang MO Ji-xian |
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| Institution: | School of Life Science, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Purpose To prepare high activity and high yield thrombin from bovine blood.MethodsUsing techniques of barium citrate absorption,ammonium sulfate fractionation and DEAE-cellulose chromatography to prepare bovine prothrombin.Prothrombin was activated by prothrombinase complex simulating the natural condition.The prothrombinase complex included coagulation factor Ⅴ,coagulation factor ,phospholipids and Ca~(2+).ResultsThe activity and yield were 85 U/mg,135 μg/mL plasma respectively.ConclusionUsing the above techniques can prepare high activity and high yield bovine thrombin on a large scale. |
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| Keywords: | coagulation factor prothrombinase complex bovine thrombin preparation |
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