| 一种促肝细胞生长因子活性测定方法 |
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| 引用本文： | 嵇扬,张润婕,王亚南.一种促肝细胞生长因子活性测定方法[J].中国生化药物杂志,2001,22(1):10-12. |
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| 作者姓名： | 嵇扬 张润婕 王亚南 |
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| 作者单位： | 解放军总后勤部 卫生部药品仪器检验所 生化室, |
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| 摘 要： | 目的建立一种新的促肝细胞生长因子 (HGF)活性测定方法。方法用原位灌流胶原酶制备大鼠肝细胞 ,经 1.5h预培养 ,将HGF加至培养上清 ,再经 1.5h孵育后 ,用溴化四唑蓝 (MTT)比色法测定细胞存活和增殖率。结果 5 71～ 1142 μg/m1HGF显著提高细胞存活和增殖率 ,1142 μg/m1HGF使A比值 (AHGF/A对照)大于2。结论此方法省时、稳定、重现性好 ,适用于HGF活性测定。
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| 关 键 词： | 促肝细胞生长因子 大鼠肝细胞 活性测定 |
| 文章编号： | 1005-1678(2001 )01-0010-03 |
| 修稿时间： | 2000年3月24日 |
A new method for the
determination of the activity of HGF |
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| Ji Yang,ZHANG Run-Jie,WANG Ya-nan.A new method for the
determination of the activity of HGF[J].Chinese Journal of Biochemical Pharmaceutics,2001,22(1):10-12. |
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| Authors: | Ji Yang ZHANG Run-Jie WANG Ya-nan |
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| Abstract: | Purpose The aim is to develop a new method for the determination of the activity of hepatocyte growth factor (HGF). Met hods Rat hepatocytes were prep ared by collagenase perfusion and incubated at 5% CO2 incubator for 1.5 h. The n HGF was added into the culture medium and incubated at 5% CO2 incubator for 1.5 h. The cell viability was determined by MTT method.Results ～1 142 μg/ml improved the number of cell viability significantly （AHGF/Acontrol） ＞2.Conclusion Th is new method was stable, time-saving and of good reappearance. It was adapted to the determination of HGF activity. |
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| Keywords: | Hepatocyte growth factor Rat hepatocytes Activity determination |
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