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聚乙二醇对溶菌酶和粒细胞集落刺激因子的初步化学修饰
引用本文:吴洁,胡卓逸,吕小斌,刘景晶.聚乙二醇对溶菌酶和粒细胞集落刺激因子的初步化学修饰[J].中国生化药物杂志,2004,25(1):8-10.
作者姓名:吴洁  胡卓逸  吕小斌  刘景晶
作者单位:中国药科大学,生命科学与技术学院,江苏,南京,210009
基金项目:江苏省社会发展基金 (BJ2 0 0 0 0 3 3 )
摘    要:目的考察聚乙二醇 (PEG)修饰对溶菌酶和粒细胞集落刺激因子 (G CSF)活性的影响。方法以不同方法活化的PEG修饰溶菌酶 ,通过正交试验和单因素考察确定合适的修饰条件 ;溶菌酶活力测定采用溶壁小球菌法 ,抗原性测定采用试管沉淀法 ;G CSF活性测定以小鼠血浆中中性粒细胞数目增殖情况反映。结果当偶联上一个PEG分子时 ,溶菌酶的活性保留 13% ,抗原性显著减弱 ;G CSF经PEG修饰后对小鼠中性粒细胞数目的增加有显著促进作用 ,且作用时间明显延长。结论PEG修饰对G CSF血循环半衰期的延长有显著作用

关 键 词:溶菌酶  粒细胞集落刺激因子  聚乙二醇  化学修饰
文章编号:1005-1678(2004)01-0008-03

Chemical modification of activated polyethylene glycol to lysozyme and granulocyte colony-stimulating factor
WU Jie,HU Zhuo-yi,LU Xiao-bing,LIU Jing-jing.Chemical modification of activated polyethylene glycol to lysozyme and granulocyte colony-stimulating factor[J].Chinese Journal of Biochemical Pharmaceutics,2004,25(1):8-10.
Authors:WU Jie  HU Zhuo-yi  LU Xiao-bing  LIU Jing-jing
Abstract:PurposeTo investigate the influence of polyethylene glycol(PEG) modification on the activity of lysozyme and granulocyte colony-stimulating factor(G-CSF).MethodsDefining the suitable condition of modification by orthogonal test and single agent test. The activity of lysozyme was tested according to the Pharmacopeia and the antigenicity tested by tube sedimentation. The activity of G-CSF was examined by injecting subcutaneously into normal mice and determining neutrophil counts at various times.Results13% of the modified enzyme activity was maintained while the antigenicity was greatly reduced. The modified G-CSF can maintain elevated neutrophil levels for extended periods.ConclusionPEG modification can elongate apparently the half life of G-CSF.
Keywords:lysozyme  granulocyte colong-stimulating factor  polyethylene glycol  chemical modification
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