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| 青黛提取物对HEL细胞增殖、凋亡和分化的影响 | |
| 引用本文： | 姜鹏君,孙雪梅,陈敏,徐祖琼,范振芳,刘志辉,陆超,朱学军.青黛提取物对HEL细胞增殖、凋亡和分化的影响[J].中国生化药物杂志,2011,32(3). |
| 作者姓名： | 姜鹏君 孙雪梅 陈敏 徐祖琼 范振芳 刘志辉 陆超 朱学军 |
| 作者单位： | 1. 南京中医药大学附属医院血液科,江苏南京210029;南京中医药大学,江苏南京210046 2. 南京中医药大学附属医院血液科,江苏南京,210029 3. 南京中医药大学附属医院中药制剂实验室,江苏南京,210029 |
| 基金项目： | 江苏省人事厅六大高峰人才项目，江苏省卫生厅科教兴卫医学重点人才，2010年国家自然科学基金 |
| 摘 要： | 目的研究青黛有效成分在体外对HEL细胞的增殖、凋亡和分化作用。方法应用MTT法检测青黛有效成分对HEL细胞的增殖抑制效应;流式细胞术检测CD14和CD11B,观察HEL细胞单核系分化趋势;流式细胞术AnnexinV-FITC/PI双标记法检测青黛有效成分对HEL细胞的早期凋亡诱导作用。结果与阴性对照组相比25,50,75和100μg/mL青黛有效成分作用下HEL细胞均出现单核系分化趋势,其中100μg/mL的分化趋势最明显;MTT结果显示25,50,75及100μg/mL药物对HEL细胞的生长抑制率分别为16.01%,39.12%,50.06%和59.28%;青黛有效成分可诱导HEL细胞凋亡:25,50,75及100μg/mL药物对HEL的早期凋亡率分别是5.60%,13.7%,17.8%和18.4%,明显高于对照细胞的自发凋亡率(2.70%)(P<0.05)。结论青黛有效成分对体外培养的HEL细胞具有显著抑制增殖作用,且可诱导细胞早期凋亡和单核系分化。 |
| 关 键 词： | 青黛 红白血病 HEL细胞 分化 凋亡 |
Study on the effect active ingredient of indigo naturalis on the proliferation,apoptosis and differentiation of human erythroleukemia (HEL) cell line |
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| JIANG Peng-jun,SUN Xue-mei,CHEN Min,XU Zu-qiong,FAN Zheng-fang,LIU Zhi-hui,LU Chao,ZHU Xue-jun.Study on the effect active ingredient of indigo naturalis on the proliferation,apoptosis and differentiation of human erythroleukemia (HEL) cell line[J].Chinese Journal of Biochemical Pharmaceutics,2011,32(3). | |
| Authors: | JIANG Peng-jun SUN Xue-mei CHEN Min XU Zu-qiong FAN Zheng-fang LIU Zhi-hui LU Chao ZHU Xue-jun |
| Institution: | JIANG Peng-jun1,2,SUN Xue-mei1,CHEN Min1,XU Zu-qiong1,FAN Zheng-fang1,LIU Zhi-hui3,LU Chao3,ZHU Xue-jun1(1.Department of Hematology,Affiliated Hospital of Nanjing University of TCM,Nanjing 210029,China,2.Nanjing University of TCM,Nanjing 210046,3.TCM Preparation Laboratory,China) |
| Abstract: | Purpose To study the effect of active ingredient of indigo naturalis on the proliferation,apoptosis and differentiation of human erythroleukemia(HEL) cell line in vitro.Methods MTT assay was employed to detect the inhibitory effect of the extraction on HEL cells.The monocytic differentiation of HEL cells was evaluated by changes of the expression of CD11B and CD14 detected by flow cytometry(FCM).And the apoptosis induction of the extract on HEL cells was demonstrated by Annexin V-FITC/PI double label method... |
| Keywords: | indigo naturalis human erythroleukemia HEL cell differentiation apoptosis |
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