| HPLC法测定金银花浸膏中绿原酸的含量 |
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| 引用本文： | 余杰,匡辽红,林凡胜,戴俊东,讯毅坤.HPLC法测定金银花浸膏中绿原酸的含量[J].中国医院用药评价与分析,2009(9):685-687. |
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| 作者姓名： | 余杰 匡辽红 林凡胜 戴俊东 讯毅坤 |
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| 作者单位： | [1]北京源生素源生物科技有限公司,北京市102212 [2]北京中医药大学中药学院,北京市100102 |
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| 摘 要： | 目的：建立金银花浸膏中绿原酸含量测定方法。方法：采用高效液相色谱法，色谱柱为Kromasil C18色谱柱（4．6mm×150mm，5μm）；流动相：乙腈-0．4％磷酸溶液（15：85）；流速：1mL·min^-1；检测波长：327nm；进样量：10皿。结果：绿原酸在0，106～1．016μg范围内呈良好的线性关系（r=0．9999），平均回收率为100．3％（RSD=1．04％）。结论：本方法操作简便，准确，重复性好，精密度高，可作为金银花浸膏的质量控制方法。
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| 关 键 词： | 金银花浸膏 绿原酸 高效液相色谱法 |
Determination of Flos Lonicera Japonica Extract by HPLC |
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| Institution: | YU Jiel, KUANG Liao-hong, LIN Fan-sheng, DAI Jun-dong , SUN Yi-kun (1 . Beijing YuanshengSuyuan Biolechnology Co. Ltd , Beijing 102212, China; 2.Beijing University of Traditional Chinese Medicine, Beijing 100102, China) |
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| Abstract: | OBJECTIVE： To establish a method for determining Chlorogenic Acid in Flos Lonicera Japonica Extract. METHODS： A Kromasil C18 column （4.6 mm x 150 mm, 5 μm） with acetonitrile -0.4% H3PO4 （15：85）as the mobile phase was used for determination, the flow rate was 1.0 mL· min ^-1 and the UV detection wavelength was at 327 nm. RESULTS：There was a good linear relationship at a range of 0. 106 - 1.016 μg for Chlorogenic Acid （r = 0.999 9）, the average recovery rate was 100.3 % with RSD of 1.04 % （n = 6）. CONCLUSION：The results indicate that the HPLC method is simple, accurate, highly selective and reproducible, thus it could be used as quality control in the preparation of Flos Lonicera Japonica Extract. |
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| Keywords: | Flos Lonicera Japonica Extract Chlorogenic acid HPLC |
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