维A酸对HL-60细胞端粒酶活性的影响

目的:探讨维A酸(ATRA)诱导HL-60细胞增殖过程中端粒酶活性的变化.方法:将不同浓度(10-6、10-7及10-8M)的ATRA处理体外培养的HL-60细胞,采用四甲基偶氮唑盐比色法(MTT)测定其细胞增殖情况,培养72 h时用TRAP-ELISA法检测端粒酶活性的变化.结果:维A酸呈时间-剂量依赖方式抑制HL-60细胞增殖,与对照组比较,实验组端粒酶活性均下降,其中10-6M及10-7M两组差异显著(P＜0.05,P＜0.01),而10-8M组无显著差异(P＞0.05).结论:维A酸能够抑制HL-60细胞增殖,该过程可能与其下调细胞端粒酶的活性有关.

Effect of All-trans Retinoic Acid on Telomerase Activity in HL-60 Cell Line

Objective:To explore the change in telomerase activity during proliferation of HL-60 cells induced by all-trans retinoic acid(ATRA).Methods:HL-60 cells were cultured in vitro with ATRA in different concentrations for different period of time.Cell viability of proliferation was assessed with MTT assay.The telomerase activity was determined with TRAP-ELISA technique 72 hours after the culture.Results:The proliferation of HL-60 cells was greatly inhibited by ATRA in a time-dosage-dependant manner.The telomerase activity was decreased after ATRA;there was a significant difference between either the concentration of 10~(-6)M group or 10~(-7)M group and the control,whereas the group of 10~(-8)M was not significantly different from the control.Conclusion:ATRA could inhibit the proliferation of HL-60 cells in vitro,and this effect minght be associated with downregulation of telomerase activity by ATRA..