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胰岛素和川芎嗪联合治疗对严重烫伤内毒素血症大鼠血浆sTREM-1、sICAM-1、MDA和SOD水平的影响
引用本文:杨洋,陈和平,王占科,祝仲珍,张晓云,王庆莅,熊圆圆.胰岛素和川芎嗪联合治疗对严重烫伤内毒素血症大鼠血浆sTREM-1、sICAM-1、MDA和SOD水平的影响[J].华北国防医药,2017,29(6).
作者姓名:杨洋  陈和平  王占科  祝仲珍  张晓云  王庆莅  熊圆圆
作者单位:1. 330006南昌,南昌大学医学院;330002南昌,解放军94医院药学科;2. 南昌大学医学院,南昌,330006;3. 330006南昌,南昌大学医学院;330002南昌,解放军94医院检验科;4. 解放军94医院感染管理科,南昌,330002;5. 解放军184医院检验科, 江西 鹰潭,335000;6. 解放军94医院检验科,南昌,330002
摘    要:目的 探讨胰岛素和川芎嗪单独和联合治疗对严重烫伤内毒素血症大鼠血浆可溶性髓样细胞触发蛋白-1(sTREM-1)、可溶性细胞间黏附分子-1(sICAM-1)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平影响,观察其抗炎和抗氧化效果.方法 建立重度烫伤内毒素血症SD大鼠模型80只,按随机数字法随机分为胰岛素组(腹腔注射胰岛素)、川芎嗪组(腹腔注射川芎嗪)、联合组(腹腔注射胰岛素和川芎嗪)及对照组(腹腔注射生理盐水),各20只.测定各组伤前,伤后1、3、5和7d血浆sTREM-1、ICAM-1、MDA和SOD水平变化.结果 4组伤后各时间点血sTREM-1和sICAM-1以及MDA水平均高于伤前(P<0.01),SOD水平低于伤前(P<0.01).胰岛素组、川芎嗪组和联合组伤后各时间点血浆sTREM-1、sICAM-1以及MDA水平均低于对照组(P<0.01),SOD水平均高于对照组(P<0.01).联合组伤后各时间点血浆sTREM-1、sICAM-1和MDA水平均低于胰岛素组和川芎嗪组(P<0.01),SOD水平均明显高于胰岛素组和川芎嗪组(P<0.01).结论 严重烫伤内毒素血症大鼠存在过度炎性反应和氧自由基损伤,胰岛素和川芎嗪具有抗炎和抗氧化作用,且联合治疗比单独治疗效果好;胰岛素和川芎嗪联合治疗可作为防治严重烫伤后过度炎性反应和氧化应激损伤的有效药物.

关 键 词:胰岛素  川芎嗪  烧伤  内毒素血症  大鼠  Sprague-Dawley  炎症  氧化性应激

Effects of Insulin Combined with Tetramethylpyrazine Therapy on Plasma sTREM-1, sICAM-1, MDA and SOD Levels of Rats with Endotoximia after Severe Scalding Injury
YANG Yang,CHEN He-ping,WANG Zhan-ke,ZHU Zhong-zhen,ZHANG Xiao-yun,WANG Qing-li,XIONG Yuan-yuan.Effects of Insulin Combined with Tetramethylpyrazine Therapy on Plasma sTREM-1, sICAM-1, MDA and SOD Levels of Rats with Endotoximia after Severe Scalding Injury[J].Medical Journal of Beijing Military Region,2017,29(6).
Authors:YANG Yang  CHEN He-ping  WANG Zhan-ke  ZHU Zhong-zhen  ZHANG Xiao-yun  WANG Qing-li  XIONG Yuan-yuan
Abstract:Objective To discuss effects of Insulin combined with Tetramethylpyrazine (TMP) therapy and every single one therapy on plasma soluble triggering receptor expressed on myeloid cells-1 (sTREM-1),soluble intercellular adhesion molecule-1 (sICAM-1),superoxide dismutase (SOD) malondialdehyde and (MDA) of rats with endotoximia after severe scalding injury,and to observe their anti-inflammatory and anti-oxidization effects.Methods A total of 80 SD rat models with endotoximia after severe scalding injury were established,and were randomly divided into Insulin group (n =20,intraperitoneal injection of Insulin),TMP group (n =20,intraperitoneal injection of TMP),combined group (n =20,intraperitoneal injection of Insulin and TMP) and control group (n =20,intraperitoneal injection of physiological saline).Changes of plasma sTREM-1,ICAM-1,MDA and SOD levels were detected before and at 1st,3rd,5th and 7th d after injury in all groups.Results In the 4 groups,STREM-1,sICAM-1 and MDA levels at all time points after injury were significantly higher (P <0.01),while SOD levels were significantly lower than those before injury (P <0.01).In Insulin group,TMP group and combined group,plasma sTREM-1,sICAM-1 and MDA levels were significantly lower (P <0.01),while SOD levels were significantly higher at all time point after injury than those in control group (P < 0.01).In combined group,plasma sTREM-1,sICAM-1 and MDA levels were significantly lower (P < 0.01),while SOD levels were significantly higher than those in Insulin and TMP groups (P < 0.01).Conclusion Rats with endotoximia after sever scalding injury have exceeding inflammatory reaction and oxygen free radical damage.Insulin and TMP have anti-inflammatory and anti-oxidization effects,and the effect of combined therapy is better than those by every single one,and therefore Insulin combined with TMP can be used as an effective medication for exceeding inflammatory reaction and oxygen free radical damage after sever scalding injury.
Keywords:Insulin  Tetramethylpyrazine  Burns  Endotoximia  Rats  Sprague-Dawley  Inflammation  Oxidative stress
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