| 足叶乙苷脂质体包封率测定方法研究 |
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| 引用本文： | 时军,陈伟鸿,程怡,林德晖.足叶乙苷脂质体包封率测定方法研究[J].临床医药实践,2010(8). |
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| 作者姓名： | 时军 陈伟鸿 程怡 林德晖 |
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| 作者单位： | 广东药学院中药学院;广州中医药大学中药学院; |
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| 基金项目： | 高等学校博士学科点专项科研基金(编号20060572012) |
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| 摘 要： | 目的:建立足叶乙苷脂质体的包封率测定方法。方法:采用乙醇注入法制备足叶乙苷脂质体,以葡聚糖凝胶分离-高效液相色谱法测定含量和包封率。结果:洗脱曲线表明采用蒸馏水(洗脱速度0.5 mL/min)作为洗脱溶剂,柱回收率为99.22%,同一批样品包封率测定结果RSD在2%以内。结论:葡聚糖凝胶分离-高效液相色谱法操作简单、准确、重复性好,可用于足叶乙苷脂质体的包封率的测定。
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| 关 键 词： | 足叶乙苷 脂质体 包封率 |
Study on Entrapment Efficiency Determination of Etoposide Liposome |
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| SHI Jun,CHEN Wei-hong,CHENG Yi,LIN De-hui.Study on Entrapment Efficiency Determination of Etoposide Liposome[J].Proceeding of Clinical Medicine,2010(8). |
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| Authors: | SHI Jun CHEN Wei-hong CHENG Yi LIN De-hui |
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| Institution: | SHI Jun,CHEN Wei-hong,CHENG Yi,LIN De-hui(Guangdong Pharmaceutical University,Cuangzhou,510006,China) |
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| Abstract: | Objective:To establish encapsulation efficiency determination method of etoposide liposome.Methods:To prepare etoposide liposome by using ethanol injection method,use gel filtration to determine the content and entrapment efficiency of etoposide liposome combining high-performance liquid chromatography.Results:The elution curve shows that the use of distilled water(flow rate 0.5mL/min) as the eluting solvent,column recovery ratio is 99.22%,encapsulation efficiency RSD of the same sample is within 2%.Conclus... |
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| Keywords: | Etoposide Liposome ntrapment efficiency |
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