外源性磷酸肌酸预处理对大鼠心肌缺血-再灌注损伤的保护作用 |
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引用本文: | 张献,纪勇,陈国强,黄斌,吴松,沈凯,虞桂平,王晓臣.外源性磷酸肌酸预处理对大鼠心肌缺血-再灌注损伤的保护作用[J].江苏医药,2011,37(14). |
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作者姓名: | 张献 纪勇 陈国强 黄斌 吴松 沈凯 虞桂平 王晓臣 |
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作者单位: | 东南大学附属江阴市人民医院胸心外科,江阴市,214400 |
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摘 要: | 目的研究外源性磷酸肌酸(CP)预处理对大鼠心肌缺血-再灌注(I-R)损伤的保护作用。方法健康雄性SD大鼠30只,随机均分为三组:假手术(S)组、I-R组和CP预处理组(CP组)。I-R、CP预处理组结扎冠状动脉左前降支30 min,再灌注120 min制备心肌I-R损伤模型。缺血前30 min,CP组经腹腔注射CP 10 ml/kg。于再灌注120 min时处死大鼠,测定心肌三磷酸腺苷(ATP)含量和超氧化物岐化酶(SOD)活性,光学显微镜和透射电镜下观察心肌组织结构变化及线粒体水肿情况。结果 CP组心肌组织中ATP含量、SOD活性显著高于I-R组(2.76±0.98)μmol/mg vs.(1.65±0.84)μmol/mg、(42.47±2.16)U/mg vs.(49.56±3.28)U/mg](P<0.05)。I-R组心肌组织明显水肿、出血和坏死;CP组心肌组织结构及线粒体变化较I-R组明显减轻。结论外源性CP对大鼠心肌I-R损伤具有明显保护作用。
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关 键 词: | 磷酸肌酸 缺血-再灌注损伤 心肌保护 |
Protective effects of exogenous creatine phosphate pretreatment on myocardial ischemia-reperfusion injury in rats |
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Abstract: | Objective To study the protective effects of creatine phosphate pretreatment on myocardial ischemia-reperfusion(I-R) injury in rats.Methods Thirty adult male SD rats were randomly and equally divided into three groups of S(sham operated),I-R(ischemia for 30 min and reperfusion for 120 min) and CP(creatine phosphate 10 ml/kg intraperitoneally injected 30 min before ischemia).Myocardial tissues were tested for adenosine triphosphate(ATP) and superoxide dismutase(SOD) activity in the myocardium were examined.M... |
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Keywords: | Creatine phosphate Ischemia-reperfusion injury Myocardium protection |
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