| 重组MMP-2对人肾系膜细胞Fkn基因表达和分泌的影响 |
| |
| 引用本文： | 刘强,方铭,王尧,查爱云,邹芳,袁扬,王艳萍,孙子林.重组MMP-2对人肾系膜细胞Fkn基因表达和分泌的影响[J].江苏医药,2011,37(3). |
| |
| 作者姓名： | 刘强 方铭 王尧 查爱云 邹芳 袁扬 王艳萍 孙子林 |
| |
| 作者单位： | 1. 东南大学医学院,南京市,210009
2. 江阴市人民医院内分泌病科 |
| |
| 基金项目： | 高等学校博士学科点专项科研基金(200802860020); 2008年无锡市科技发展指导性计划项目(csz00804) |
| |
| 摘 要： | 目的观察重组基质金属蛋白酶-2(MMP-2)对人肾系膜细胞(HRMC)趋化因子Fkn mRNA表达的影响。方法用重组MMP-2干预体外培养的HRMC,逆转录PCR检测Fkn mRNA表达,ELISA法检测HRMC上清液中Fkn蛋白含量。结果 HRMC的Fkn mRNA表达水平随MMP-2浓度增加而增加(P<0.05),上清液中Fkn蛋白含量随MMP-2浓度增加而降低(P<0.05)。结论重组MMP-2可以增加Fkn mRNA的表达,而降低上清液中Fkn蛋白含量。
|
| 关 键 词： | 基质金属蛋白酶-2 趋化因子Fkn基因 人肾系膜细胞 |
Effects of MMP-2 on the expression of fractalkine in human renal mesangial cells |
| |
| LIU Qiang,FANG Ming,WANG Yao,et al..Effects of MMP-2 on the expression of fractalkine in human renal mesangial cells[J].Jiangsu Medical Journal,2011,37(3). |
| |
| Authors: | LIU Qiang FANG Ming WANG Yao |
| |
| Institution: | LIU Qiang,FANG Ming,WANG Yao,et al.School of Medicine,Southeast University,Nanjing 210009,CHINA |
| |
| Abstract: | Objective To observe the effects of matrix metalloproteinase-2(MMP-2) on the expression of fractalkine(Fkn) in cultured human renal mesangial cells(HRMCs).Methods HRMCs were incubated with different concentrations of MMP-2.Fkn mRNA expression of HRMCs was analyzed by RT-PCR and the concentration of Fkn in the supernatant was quantified by ELISA.Results Fkn mRNA expression of HRMCs increaed as MMP-2 increased (P<0.05).The expression of Fkn protein in the supernatant decreased as MMP-2 increased(P<0.05).Concl... |
| |
| Keywords: | Metalloproteinase-2 Fractalkine Human renal mesangial cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
