Water Soluble Components of ‘Osteocare’ Promote Cell Proliferation,Differentiation, and Matrix Mineralization in Human Osteoblast-Like SaOS-2 Cells |
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| Authors: | Sandeep R Varma L M Sharath Kumar Satyakumar Vidyashankar Pralhad Sadashiv Patki |
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| Institution: | 1.Department of Cell Biology, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.;2.Department of Phytochemistry, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India.;3.Medical Services and Clinical Trials, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India. |
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| Abstract: | Osteocare, a herbal formulation, has been found to be very effective in bone mineralization and support of the microstructure of bone tissue. The water-soluble components of Osteocare (WSCO) induced osteogenic activity in human osteoblast-like SaOS-2 cells. The addition of WSCO (100 μg/ml) to SaOS-2 cells was effective in increasing the cell proliferation by 41.49% and DNA content by 1.9-fold. WSCO increased matrix mineralization in SaOS-2 cells by increased alkaline phosphatase levels and calcium-rich deposits as observed by Alizarin red staining. WSCO markedly increased mRNA expression for osteopontin (OPN), osteocalcin (OCN), type I collagen (Col I) in SaOS-2 cells, and it down-regulated IL-6 mRNA levels in SaOS-2 cells. The present study showed that WSCO plays an important role in osteoblastic bone formation through enhanced activities of ALP, Col I, bone matrix proteins such as OPN and OCN, down-regulation of cytokines like IL-6, as well as promoting mineralization in SaOS-2 cells. |
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| Keywords: | Osteocare Cell proliferation ALP activity OCN OPN Col-I IL-6 Mineralization |
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