独角莲醇提液对H22 肝癌荷瘤小鼠血清IL-2和TNF-α水平的影响

目的探讨独角莲醇提液对H22肝癌荷瘤小鼠血清IL-2和TNF-α水平的影响。方法建立体内H22肝癌荷瘤小鼠的模型，采用灌胃法给予其100mg·kg^-1·d^-1独角莲醇提液，以生理盐水灌胃和5-FU腹腔注射作为对照，连续10d，停药后第2天摘取小鼠眼球取血后断髓处死，取出胸腺、脾脏称重，计算小鼠的胸腺重量指数、脾脏重量指数，采用ELISA法检测小鼠血清中细胞因子白介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）的水平。结果与生理盐水灌胃的H22肝癌荷瘤小鼠比较，5-FU腹腔注射和独角莲醇提液灌胃处理的H22荷瘤鼠血清中细胞因子IL-2、TNF-α水平明显升高；但胸腺重量指数、脾脏重量指数显著下降，差异均具有统计学意义（P〈0．05）。结论独角莲醇提液可提高H22荷瘤鼠的血清IL-2和TNF-α水平。

--------------------------------

Objective To explore the effects of alcohol extracts of Typhonium giganteum Engl. on the serum IL-2 and TNF-alpha levels of H22 liver cancer-bearing mice. Methods The models of in vivo H22 cancer bearing mice were estabhshed, and 10 nfice were lavaged with 100 mg.kg^-1.d ^-1 of alcohol extracts of Typhonium giganteum Engl., and another 10 had intra- peritoneal injection of 5-FU, and the remaining 10 had lavage of saline and were taken as control, for 10 days. On the 2nd day after drug discontinuation, the mice were pulpotomy executed after being taken blood from eyes, and then the thymus, spleen were removed, weighed and calculated. The serum interleukin-2 （IL-2） and tumor necrosis factor-alpha （TNF-α ） levels were detected by ELISA. Results Compared with the mice lavaged by saline, the serum IL-2 and TNF-α levels of mice with intra- peritoneal injection of 5-FU and those with Typhonium giganteum Engl. alcohol extracts significantly increased, but their thymus weight index, spleen weight index both decreased （P〈 0.05）. Differences all had statistical significance. Conclusion The alcohol extracts ofTyphonium giganteum Engl. could enhance serum IL-2 and TNF-α levels of H22 hver cancer-bearing mice.