水飞蓟宾对小鼠肝脏炎症损伤和肿瘤坏死因子的产生及活性的影响

建立了小鼠肝损伤模型及体内外肿瘤坏死因子(TNF)诱生的方法,研究水飞蓟宾(SB)对肝脏炎症损伤、TNF产生及其生物活性的影响。结果表明∶SB在体内外均可显著抑制脂多糖(LPS)诱导TNF产生;在体外能抑制TNF对肝细胞系GSG-7701和成纤维细胞系L929的细胞毒作用;在体内对LPS诱导的痤疮丙酸杆菌致敏的小鼠肝脏炎症损伤有保护作用。提示SB的保肝作用机理与其抑制TNF的产生和活性有关。

EFFECT OF SILYMARIN ON MOUSE LIVER DAMAGE, PRODUCTION AND ACTIVITY OF TUMOR NECROSIS FACTOR

Tumor necrosis factor (TNF) has been well characterized as a prominent mediator in the development of liver injury. Effects of silymarin(SB) on mouse liver damage, TNF production and activity were studied. Pretreatment with SB(25～50 mg·5kg^-1, ip, bid×3 d) before the lipopolysaccharides (LPS) injection markedly alleviated liver injury and diminished LPS-induced TNF production in Propionibacterium acnes (PA) primed mice. SB(12.5～50 μg·5mL^-1 ) significantly inhibited LPS-induced TNF release from mouse peritoneal macrophage in a concentration dependent manner. SB(12.5～100 μg·ml^-1 ) was also shown to markedly reduce TNF cytotoxicity on human hepatic cell line GSG-7701 and mouse fibroblastic cell line L929 cells concentration dependently. These results suggest that inhibition of TNF production and its actions may be involved in the mechanism of protective action of SB on liver damage.