环氧司坦对胎盘滋养层、黄体细胞及胚胎与子宫内膜共培养的影响

环氧司坦(2-氰基-3,17 α-二羟基-4β,17β-二甲基-4α,5α-环氧-雄甾烯(epostane)被认为是颇有希望的止孕药物,国外已应用于临床,为了进一步探讨环氧司坦的止孕机理,我们研究了它对离体人胎盘滋养层细胞和豚鼠黄体细胞的直接作用及对hCG的促黄体激素生成作用和小鼠胚胎与子宫内膜“共培养”的影响。

EFFECTS OF EPOSTANE ON CHORIO-TROPHOBLAST, COCULTURE OF EMBRYOS WITH ENDOMETRIUM AND CORPORA LUTEA IN VITRO

Effects of epostane on human trophoblast cells, co-culture of mouse embryos with endometrium and guinea pig corpora lutea were studied in vitro. Human chorio-trophoblast cells were obtained from induced abortion (2-2.5 months of pregnancy). The tissue was incubated in control medium or in medium containing epostane. The results indicated that epostane at a concentration of 50 micrograms/ml could injure the human trophoblast cells and its function. Obviously damaged cells were observed at the concentration of 100 micrograms/ml. The cells were degenerated with pyknotic nuclei and sometimes only cell fragments were found. Guinea pig corpora lutea produced progesterone in vitro. In the incubation medium of the control group, the total luteal progesterone increased linearly with the prolongation of incubation during the first 30 min of incubation. The addition of hCG at concentration of 10 IU/ml clearly accelerated progesterone production. Epostane at a concentration of 50 micrograms/ml could significantly inhibit both basic and the hCG-stimulated luteal production of progesterone. Epostane at concentrations of 50 and 100 micrograms/ml showed no inhibitory effect on mouse embryos and endometrium in vitro.