光纤化学传感仪实时监测利福平胶囊溶出度

目的研究建立连续、在线和自动化的实时监测利福平胶囊溶出度的方法。方法设计了光纤化学传感器仪器系统,利用利福平与荧光分子探针4-(N,N-二辛基)氨基-7-硝基苯-2-氧-1,3-二氮杂茂(NDB)的可逆响应,基于荧光多元猝灭原理,在Windows 98平台下实时监测利福平胶囊的溶出度。结果利福平的浓度在10～170 μg·mL^-1与Ln(F₀/F)成线性关系,相关系数为0.9993,实时监测利福平胶囊的溶出度,可直接从溶出曲线上提取相关参数,与药典方法测定相比无显著性差异(P>0.05)。结论本法获得的数据信息完整,真实反映了药物在体外的溶出过程。相应软件的设计实现了药物溶出度监测的智能化。

A FIBER OPTIC CHEMICAL SENSOR SYSTEM FOR ON-LINE MONITORING THE DRUG DISSOLUTION OF RIFAMPICIN

AIM: To study a continuous, on-line and automatic monitoring method for dissolution of drugs. METHODS: A new fiber optical chemical sensor prepared by sol-gel immobilization of molecular probe was reported. Based on multiple quenching of flurescence, an instrument and software system was designed to on-line monitor the drug dissolution of rifampicin. RESULTS: The concentration of rifampicin was linear with ln(F0/F) when concentration was in the range of 10-170 micrograms/mL-1. The relative coefficient was 0.9993. The procession of dissolution can be on-line monitored. Parameters were obtained directly from the dissolution curve. There is no distinctly difference when compared with the method of the Pharmacopoeia of People's Republic of China (P > 0.05). CONCLUSION: This procession analysis can reflect the real dissolution of drug and obtain the total information.