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胶束色谱分析黄连及含黄连中成药中小檗碱型生物碱的研究
引用本文:邱晓星,伍朝筼,陈柏林.胶束色谱分析黄连及含黄连中成药中小檗碱型生物碱的研究[J].药学学报,1986,21(6):458-465.
作者姓名:邱晓星  伍朝筼  陈柏林
作者单位:华西医科大学药学院 成都 (邱晓星,伍朝筼),中国科学院成都分院分析测试中心 北京医科大学药学院分析教研室(陈柏林)
摘    要:本文报道用胶束色谱法分离测定黄连(黄柏)及含黄连(黄柏)中成药中黄连碱、药根碱、巴马亭和小槃碱。考察了固定相、胶束浓度、有机改性剂浓度及pH对组分保留行为的影响。实验结果表明;影响该色谱分离的主要因素为固定相极性和胶束浓度。同时在肢束流动相中加入甲醇改性剂,可在室温下显著地提高柱效。用键合苯基固定相,以3.5%十二烷基硫酸钠/0.1 N酒石酸—甲醇(30:70)胶束溶液作流动相,测定了十几种黄连(黄柏)及含黄连(黄柏)的中成药。

关 键 词:胶束色谱  黄连  黄柏  中成药  黄连碱  巴马亭  药根碱  小檗碱
收稿时间:1985-09-04

ANALYSIS OF BERBERINE-TYPE ALKALOIDS IN RHIZOMA COPTIDIS AND CHINESE PATENT MEDICINES BY MICELLAR CHROMATOGRAPHY
QIU Xiao-Xing,WU Chao-Yun and CHEN Bai-Lin.ANALYSIS OF BERBERINE-TYPE ALKALOIDS IN RHIZOMA COPTIDIS AND CHINESE PATENT MEDICINES BY MICELLAR CHROMATOGRAPHY[J].Acta Pharmaceutica Sinica,1986,21(6):458-465.
Authors:QIU Xiao-Xing  WU Chao-Yun and CHEN Bai-Lin
Abstract:Micellar chromatography was chosen for the separation of coptisine, jatrorrhizine, palmatine and berberine. Several factors affecting the separation selectivity of solutes——stationary phase, concentration of micelle, ratio of organic modifier and pH——were investigated. Experimental evidences indicate: the main effective factors are the polarity of stationary phase and the concentration of micelle. At room temperature, the efficiency of micellar chromatography can be improved only by adding methanol into the mobile phase. A reversed-phase system of μ-Bondapakphenyl column with a micellar solution of 3.5% sodium dodecyl sulfate/0.1 N tartaric acid-methanol (30:70) as mobile phase was used to determine coptisine, jatrorrhizine, palmatine and berberine in 8 rhizoma Coptidis and cortex Phellodendri and in 8 Chinese patent medicines.
Keywords:Micellar chromatography  Rhizoma Coptidis  Cortex Phellodendri  Chinese patent medicine  Coptisine  Palmatine  Jatrorrhizine  Berberine
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