苦皮藤根皮的1HNMR指纹图解析

目的对苦皮藤的¹HNMR指纹图进行解析.方法用硅胶柱色谱法分离苦皮藤根皮CGEA的化学成分,鉴定单体化合物的结构并对其进行¹HNMR研究,从而实现苦皮藤根皮的¹HNMR指纹图解析.结果不同来源的苦皮藤样品,其¹HNMR指纹图有很好的重现性和高度的特征性.从其CGEA中分得3个主要成分,经光谱分析鉴定其结构,分别为:angulatinA(1),angulatinB(2)和angulatinC(3).结论苦皮藤根皮的¹HNMR指纹图可用于其基源鉴定,并主要显示了以上3个化合物的特征共振信号;化合物3为新化合物,化合物2为首次从苦皮藤分离得到.

ASSIGNMENTS OF 1HNMR FINGERPRINT OF THE ROOT BARK OF CELASTRUS ANGULATUS

AIM To assign the 1HNMR finger print of the root bark of Celastrus angulatus. METHODS Silica gel column chromatography was used to separate the chemical constituents of CGE A of the root bark of C.angulatus. The characteristic signals of the 1HNMR finger print were assigned after determining the structures of the compounds isolated from CGE A. RESULTS 1HNMR fingerrints of the samples of C.angulatus collected from different regions showed highly characteristic features and reproducibility. Three compounds predominant in CGE A were isolated and their structures were determined by spectral analysis as: angulatin A (1), angulatin B (2) and angulatin C (3). CONCLUSION Compound 3 is a new compound. Compound 2 was isolated from C.angulatus for the first time. The 1HNMR finger print of CGE A of the root bark of C.angulatus showed mainly the characteristic signals of the above three compounds and might be utilized for the original authentication of this plant.