核磁共振法测定喃氟啶温度敏感性脂质体的相转变温度

用核磁共振法测定了喃氟啶温度敏感性脂质体的相转变温度。此法与经典的差热分析法不同,有灵敏度高、准确性好、提供信息全面等优点。用该法测得的DPPC-脂质体和DSPC-脂质体的相转变温度分别为36℃和48℃;DPPC-DSPC-脂质体的相转变温度与DPPC和DSPC的含量有关,随DPPC含量的增加而降低,随DSPC含量的增加而增加;药物的加入及含量不影响相转变温度。同时,制得可供临床前研究用相转变温度为41℃的喃氟啶温度敏感性脂质体。

DETERMINATION OF LIQUID-CRYSTALLINE TRANSITION TEMPERATURE OF TEMPERATURE-SENSITIVE LIPOSOMES OF FUTRAFUL BY 1HNMR

1HNMR was employed to determine the liquid-crystalline transition temperature (TC) of the temperature-sensitive liposomes of futraful. It is a method with high sensitivity, high accuracy and furnishing more information. The 1HNMR spectra were made and studied. The rate of peak height increase of phospholipid correlates to the thermotropic phase transition of liposomes. The study shows that the TC of DPPC-liposomes and DSPC-liposomes is 36 degrees C and 48 degrees C, respectively. The TC of DPPC-DSPC-liposomes correlates with the proportion of the amount of DPPC and DSPC. The TC is not affected by futraful. The temperature-sensitive liposomes of futraful with TC of 41 degrees C were prepared which consisted of 60% DPPC and 40% DSPC.