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谷氨酸诱导体外培养的鸡胚脊髓神经细胞释放NO
引用本文:林凌,周峰泉,汤国枝,张鹤云,张祖暄.谷氨酸诱导体外培养的鸡胚脊髓神经细胞释放NO[J].药学学报,1997,32(1):19-22.
作者姓名:林凌  周峰泉  汤国枝  张鹤云  张祖暄
作者单位:南京大学医学院,南京大学生化系
摘    要:用鸡胚脊髓神经细胞的原代培养,测定细胞中亚硝酸盐的含量,研究了谷氨酸(Glu)对原代培养神经细胞中NO的影响。结果表明,谷氨酸作用于原代培养的鸡胚脊髓神经细胞,在诱导神经细胞释放乳酸脱氢酶(LDH)的同时,还可诱导细胞释放一氧化氮(NO)。如先用NO合成酶抑制剂L-NOARG作用细胞,再加入Glu,则发现L-NOARG能降低Glu导致的培养液中LDH活性升高。提示NO可能参与介导Glu的神经毒性作用。

关 键 词:谷氨酸  脊髓神经细胞  一氧化氮  一氧化氮合成酶抑制剂
收稿时间:1996-01-16

GLUTAMATE CAUSES RELEASE OF NITRIC OXIDE FROM CHICKEN SPINAL CORD IN VITRO
L Lin,FQ Zhou,GZ Tang,HY Zhangand ZX Zhang.GLUTAMATE CAUSES RELEASE OF NITRIC OXIDE FROM CHICKEN SPINAL CORD IN VITRO[J].Acta Pharmaceutica Sinica,1997,32(1):19-22.
Authors:L Lin  FQ Zhou  GZ Tang  HY Zhangand ZX Zhang
Institution:School of Medicine, Nanjing University, Nanjing 210093.
Abstract:Nitric oxide (NO) has been identified to have profound effects on many systems, especially the neural tissues. Many studies suggest that NO mediate the neurotoxicity of glutamate (Glu). In order to determine in vitro whether Glu causes release of NO from chicken spinal cord, different concentrations of Glu (0.1, 0.5, 1.0 mmol.L-1) were added to the primary cultured chicken spinal cord cells, and the quantity of NO- (the metabolism product of NO) in medium was detected. The result shows that Glu can enhance obviously the concentration of NO in primary cell cultures (212% compared with the control). If the spinal cord cells were pretreated with NO synthases (NOS) inhibitor--L-NOARG, the NO-] was decreased compared with those treated with Glu only, and, on the contrary, the viability of cells was increased. All of the results above indicate that NO may play an important role in the neurotoxicity of Glu on nervous cells. It might be that Glu can instigate the activity of NO synthases, then induce a series of changes within cell and finally lead to the toxic effect on cells.
Keywords:Nitric oxide  Glutamate  Spinal neurons  Nitric oxide synthases inhibitor
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