白木通多糖的研究

用碱液从白木通(Akebia trifoliate)茎中提取所得的粗多糖ATB经DEAE-Celulose及SephadexG-200柱层析后得到一多糖纯品ATBB 2,其分子量为2.3×10⁵。糖组分分析ATBB-2中各糖残基的摩尔比为Rha∶Ara∶Xyl∶Gal∶Glc∶GalA=1.22∶1.00∶1.10∶0.85∶0.24∶0.82。经甲基化,高碘酸氧化,Smith降解,部分酸水解,¹H和¹³CNMR谱的分析揭示ATBB-2是一个结构复杂的杂多糖。

STUDIES ON POLYSACCHARIDE FROM AKEBIA TRIFOLIATE (I)

Crude polysaccharide ATB was extracted from the stems of Akebia trifoliate with 5% NaOH. It was then subjected to DEAE-Cellulose column chromatography and purified by Sephadex G-200 chromatography. Finally, a pure polysaccharide ATBB-2 was obtained. The molecular weight of ATBB-2 was estimated to be 2.3 x 10(5). By total acid hydrolysis, the analytical results indicated that ATBB-2 is composed of Rha, Ara, Xyl, Gal, Glc and GalA in a molar ratio of 1.22:1.00:1.10:0.85:0.24:0.82. Methylation analysis, periodate oxidation analysis, Smith degradation, partial acid hydrolysis, 1H and 13CNMR showed that ATBB-2 is a highly branched molecule without a main chain.