穿山甲化学成分的研究

自中药穿山甲(Manis pentadactyla Linnaeus)中分离得到七个化合物(Ⅰ～Ⅶ),分别鉴定为硬脂酸(Ⅰ)、胆甾醇(Ⅱ)、二十三酰丁胺(Ⅲ)、碳原子数为26和29的二个脂肪族酰胺(Ⅳ～Ⅴ)、L-丝-L-酪环二肽cyclo-(L-seryl-L-tyrosyl)](Ⅵ)和D-丝-L-酪环二肽cyclo-(D-seryl-L-tyrosyl)](Ⅶ),其中Ⅵ和Ⅶ为非对映立体异构体(diastereoisomers),系首次从天然物中得到,化合物Ⅶ为一新的化合物。化合物Ⅵ和Ⅶ的分子结构均经X-衍射方法得到证明。穿山甲含18种微量元素,其水溶液含16种游离氨基酸。分析比较炮制前后的穿山甲化学成分,表明两者基本相同。

STUDIES OF CHEMICAL CONSTITUENTS OF MANIS PENTADACTYLA

Seven constituents (Ⅰ～Ⅶ) were isolated from the Chinese traditional drug, Chuanshanjia (the scales of Mania pentadactyla Linnaeus). They were identified as stearic acid(Ⅰ), cholesterol(Ⅱ), N-butyltricosylamide(Ⅲ), two aliphatic amides, one consisting of 26 carbons(Ⅳ) and the other of 29 carbons(Ⅴ), cyclo(L-seryl-L-tyrosyl)(Ⅵ) and cyclo-(D-seryl-L-tyrosyl) (Ⅶ). Ⅵ and Ⅶ are diastereoisomers separated from natural resources for the first time. Ⅶ is a new compound. The stereostructures of cyclo-(L-seryl-L-tyrosyl) and cyclo-(D-seryl-Ltyrosyl) were further confirmed by X-ray diffraction analysis. Sixteen kinds of free amino acids in the water solution of the drug and eighteen trace elements in the drug were analyzed qualitatively and quantitatively.The chemical constituents of the processed sample and the unprocessed sample of Chuanshanjia were compared with the help of TLC and HPLC. The results showed that both samples contain almost the same constituents.