商陆多糖Ⅰ联合白细胞介素2对小鼠脾细胞杀瘤活性的影响

商陆多糖Ⅰ(PAP-I),0.3～3μg·ml^-1和小鼠脾细胞培养3～5d可显著增强其杀伤P815肿瘤细胞活性及IL-2(250～500IU·ml^-1)诱导的LAK细胞活性,最适浓度为1μg·ml^-1。PAP-I及IL-2和脾细胞培养的上清液对P815肿瘤细胞无细胞毒作用,但能增强脾细胞及LAK细胞杀瘤活性。PAP-I,5,10及50mg·kg-1,ip可增强脾细胞杀伤P₈₁₅和L₉₂₉细胞的活性及IL-2诱导的LAK细胞活性。

EFFECTS OF PHYTOLACCA ACINOSA POLYSACCHARIDESI COMBLNED WITH INTERLEUKIN-O ON THE CYTOTOXICITY OF MURINE SPLENOCYTES AGAINST TUMOR CELLS

Phytolacca acinosaccharides I(PAP-I),a kind of purified polysac- charides,isolated from Phytolacca acinosa Roxb was found to significantly augment the cyto- toxicity of murine splenocytes and interleukin-2( IL-2)activated splenocytes against P₈₁₅ tumor cells in uitro. The optimal concentration of PAP-I was 1μg·ml^-1 and the peak level of the cytotoxicity against P815 tumor cells was reached on d 3～5.The supernatants collected from splenocytes cultured with PAP-I alone or in combination with IL-2 showed no effect on the cytotoxicity against P815 tumor cells.Splenocytes from mice injected ip with PAP-I,5, 10 and 50 mg·kg^-1 ,thrice a week produced more cytotoxicity against P815 and L929 tumor cells compared with the control group.PAP-I ip was shown to significantly increase IL-2 activated killer cell activity(LAK)against P815 tumor cells.The higher the dosage of PAP-I,the more potent the LAK activity was observed.These results confirmed that PAP-I can augment the cytotoxicity of murine splenocyte against tumor cells and LAK activity and warranted further evaluation of its clinical usefulness.