| 钙离子对4-氨基吡啶诱发肥大细胞释放组胺作用的影响 |
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| 引用本文： | 徐建华,王佩,陈志刚,张剑,.钙离子对4-氨基吡啶诱发肥大细胞释放组胺作用的影响[J].药学学报,1995,30(3):235-238. |
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| 作者姓名： | 徐建华 王佩 陈志刚 张剑 |
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| 作者单位： | 浙江医科大学药学系药理学教研室,杭州 310006 |
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| 摘 要： | 4-Aminopyridine(4-AP) has been shown to induce histamine release fromisolated peritoneal mast cells(PMC)in mice and rats. In the presence of extracellular calcium atnormal level(0.9 mmol·L-1)histamine release induced by 4-AP(13. 6 mmol·L-1) from mice PMCwas 33.0%±4.6%,while at low calcium concentration (0.5 mmol·L-1) and in calcium-freemedium this parameter decreased to 25.5%±4.2%and 16.3%±3.7%respectively.Histaminerelease in response to 4-AP(10 mmol·L-1)from rat PMC was 39.1%±6.7%(0.9 mmol·L-1calcium),while at low calcium concentration(0. 5 mmol·L-1) and in calcium-free medium thisparameter decreased to 29.3%±4.7%and 20.2%±2.9% respectively. Results of statisticalanalysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMCwere preincubated in calcium-free medium with EDTA 0.1 mmol·L-1 for 180 min 4-AP inducedhistamine release was 13.8%±1.6%.This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells.The mechanism of 4-AP induced histamine release was discussed.
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| 关 键 词： | 钙离子 4-氨基吡啶 肥大细胞 组胺释放 |
| 收稿时间： | 1994-08-01 |
INFLUENCE OF CALCIUM CONCENTRATION ON 4-AMINOPYRIDINE INDUCED HISTAMINE RELEASE FROM ISOLATED PEKITONEAL MAST CELLS |
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| JH Xu,P Wang,ZG Chen and JX Zhang.INFLUENCE OF CALCIUM CONCENTRATION ON 4-AMINOPYRIDINE INDUCED HISTAMINE RELEASE FROM ISOLATED PEKITONEAL MAST CELLS[J].Acta Pharmaceutica Sinica,1995,30(3):235-238. |
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| Authors: | JH Xu P Wang ZG Chen and JX Zhang |
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| Abstract: | 4-Aminopyridine(4-AP) has been shown to induce histamine release fromisolated peritoneal mast cells(PMC)in mice and rats. In the presence of extracellular calcium atnormal level(0.9 mmol·L-1)histamine release induced by 4-AP(13. 6 mmol·L-1) from mice PMCwas 33.0%±4.6%,while at low calcium concentration (0.5 mmol·L-1) and in calcium-freemedium this parameter decreased to 25.5%±4.2%and 16.3%±3.7%respectively.Histaminerelease in response to 4-AP(10 mmol·L-1)from rat PMC was 39.1%±6.7%(0.9 mmol·L-1calcium),while at low calcium concentration(0. 5 mmol·L-1) and in calcium-free medium thisparameter decreased to 29.3%±4.7%and 20.2%±2.9% respectively. Results of statisticalanalysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMCwere preincubated in calcium-free medium with EDTA 0.1 mmol·L-1 for 180 min 4-AP inducedhistamine release was 13.8%±1.6%.This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells.The mechanism of 4-AP induced histamine release was discussed. |
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| Keywords: | Ca2+ 4-Aminopyridine Mast cell Histamine release |
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