利巴韦林在体外对小鼠脾细胞NK活性的影响

采用¹²⁵I-UdR同位素释放试验,观察利巴韦林(三氮唑核苷,RTC)在体外对小鼠脾细胞自然杀伤(Nk)活性的影响。结果表明,RTC的剂量与NK活性呈负相关,在5umol·L^-1时RTC即可显著抑制NK活性。RTC与地塞米松合用对NK活性的抑制有相加作用,而RTC与γ干扰素合用则互为拮抗作用。此外,还发现RTC能明显降低刀豆蛋白A诱生的小鼠脾细胞干扰素及白细胞介素-2的产量,推测RTC对NK活性抑制的部分机理是阻碍了这些淋巴因子的生成。

EFFECT OF RIBAVIRIN ON NK ACTIVITIES OF MURINE SPLEEN CELLS IN VITRO

The effects of ribvirin(1-β-D-ribofuranosy 1-1,2,4-triazole-3-carboxamide,RTC)on natural killer(NK)activities of murine spleen cells in vitro were studied by¹²⁵I-UdR isotoperelease assay.The results showed that RTC at the concentration of 5 umol·L^-1 significantlysuppressed NK activities(P<0.01),and a negative correlation between NK activities and RTCdosage was obtained.It also indicated that there was additive effect between the actions of RTC anddexamethasone(Dex,1 umol·L^-1 ),and antagonism between the actions of RTC and interferon-γ(100 U·ml^-1 ).In addition,RTC at the concentration of 0.5 umol·L^-1 was found to be enough toreduce the product of interferon(P<0.01)and interieukin-2(P<0.05)induced by Con A in murinespleen cells,and a negative dcoe-effect relationship was obeerved.Thus,one of the mechanisms ofRTC in inhibiting NK activities seems to suppress the production of these lymphokines.